Transcript: Human NM_014612.5

Homo sapiens family with sequence similarity 120A (FAM120A), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
FAM120A (23196)
Length:
5160
CDS:
232..3588

Additional Resources:

NCBI RefSeq record:
NM_014612.5
NBCI Gene record:
FAM120A (23196)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_014612.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000346070 ATAGGCCAGTTCGTCAGTATG pLKO_005 2036 CDS 100% 10.800 15.120 N Fam120a n/a
2 TRCN0000074922 GCCTTGAATAATGACTCTAAA pLKO.1 3469 CDS 100% 13.200 10.560 N FAM120A n/a
3 TRCN0000074919 CGTGCAATACAAATCCTCATT pLKO.1 3491 CDS 100% 4.950 3.960 N FAM120A n/a
4 TRCN0000074921 GCTTCCTTTCACTGGAGTTTA pLKO.1 982 CDS 100% 13.200 9.240 N FAM120A n/a
5 TRCN0000074920 GCTGACTATGTACGCAACATT pLKO.1 1090 CDS 100% 5.625 3.938 N FAM120A n/a
6 TRCN0000074918 GCTTTCTTTAAGGCTTTGATT pLKO.1 4021 3UTR 100% 5.625 3.938 N FAM120A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_014612.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02734 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02734 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000480694 ATACAGGGTGCACGCCTTGCGCGT pLX_317 13.6% 100% 100% V5 n/a
4 ccsbBroadEn_07851 pDONR223 100% 99.9% 100% None 762C>T;1851A>G n/a
5 ccsbBroad304_07851 pLX_304 0% 99.9% 100% V5 762C>T;1851A>G n/a
6 TRCN0000476487 TTGCCACTAGACTTATAACTTATA pLX_317 9.1% 99.9% 100% V5 762C>T;1851A>G n/a
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