Construct: ORF TRCN0000476487
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005640.1_s317c1
- Derived from:
- ccsbBroadEn_07851
- DNA Barcode:
- TTGCCACTAGACTTATAACTTATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM120A (23196)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476487
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23196 | FAM120A | family with sequence simila... | NM_014612.5 | 99.9% | 100% | 762C>T;1851A>G |
| 2 | human | 23196 | FAM120A | family with sequence simila... | NM_001286722.2 | 99.8% | 99.9% | 762C>T;1028_1029insAGC;1848A>G |
| 3 | human | 23196 | FAM120A | family with sequence simila... | XM_005251842.5 | 99.8% | 99.9% | 762C>T;1851A>G;2802_2803insGGC |
| 4 | human | 23196 | FAM120A | family with sequence simila... | XM_011518412.2 | 97.4% | 97.4% | 762C>T;1419_1502del;1935A>G |
| 5 | human | 23196 | FAM120A | family with sequence simila... | XM_011518413.2 | 97.4% | 97.3% | (many diffs) |
| 6 | human | 23196 | FAM120A | family with sequence simila... | XM_011518414.3 | 97.4% | 97.3% | (many diffs) |
| 7 | human | 23196 | FAM120A | family with sequence simila... | XM_011518416.3 | 66.7% | 66.3% | (many diffs) |
| 8 | human | 23196 | FAM120A | family with sequence simila... | NM_001286723.1 | 57.7% | 56.9% | (many diffs) |
| 9 | human | 23196 | FAM120A | family with sequence simila... | NM_001286724.2 | 54.1% | 52.8% | (many diffs) |
| 10 | human | 445577 | C9orf129 | chromosome 9 open reading f... | NM_001098808.1 | 14.6% | 12.3% | (many diffs) |
| 11 | human | 445577 | C9orf129 | chromosome 9 open reading f... | XM_006717117.4 | 14.6% | 12.3% | (many diffs) |
| 12 | human | 445577 | C9orf129 | chromosome 9 open reading f... | NM_001368813.1 | 10.8% | 10.7% | 0_1ins1053;251T>C;366_366delGins1936 |
| 13 | mouse | 218236 | Fam120a | family with sequence simila... | NM_001033268.2 | 91.4% | 96.4% | (many diffs) |
| 14 | mouse | 218236 | Fam120a | family with sequence simila... | XR_001780774.1 | 69.4% | (many diffs) | |
| 15 | mouse | 218236 | Fam120a | family with sequence simila... | XR_001780776.1 | 64.4% | (many diffs) | |
| 16 | mouse | 218236 | Fam120a | family with sequence simila... | XR_001780775.1 | 58% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3423
- ORF length:
- 3354
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcgtgcag ggcttccagg actacatcga gaagcactgc ccgagcgccg 121 tggtgccggt ggagctgcag aagctggccc ggggcagcct ggtgggcggc gggcggcagc 181 ggcccccgca gaccccgctg cgcctgctgg tggacgccga caactgcctg caccgcctct 241 acggcggctt ctacaccgac tgggtcagcg gcggccagtg gaaccacatg cttggctacc 301 tggcggcgct ggccaaggcc tgcttcggcg gcaacatcga gctcttcgtc ttcttcaacg 361 gcgcgctcga gaaggcccgg ctgcacgagt gggtcaagcg gcagggcaac gagcgccaga 421 cggcacagca gatcgtcagc catgtccaga acaagggcac cccgccgcca aaggtctggt 481 tcctgccgcc cgtctgcatg gcccactgca tccgcctggc gctcatccgc ttccacgtca 541 aggttgcaca gagcattgag gatcaccatc aggaagtgat tggtttctgc agagagaatg 601 gtttccatgg cttggttgcg tatgactctg attatgcact gtgcaacatc ccctactatt 661 tcagtgccca tgccctaaaa ctgagccgga acgggaaaag tctcaccaca agccaatatc 721 tgatgcatga agttgccaag caactggacc tgaatccaaa tcgttttcct atttttgctg 781 ctctcttagg aaatcacatt ctgcctgatg aagatctggc ttcctttcat tggagtttac 841 ttggtccaga acatccacta gcctcactaa aggtccgggc ccaccagctg gtcttgccac 901 cttgcgacgt agtgatcaaa gccgttgctg actatgtacg caacattcag gacacctctg 961 acttggatgc catagctaaa gatgttttcc agcattcaca gtctagaaca gatgacaaag 1021 ttattcgatt taagagagca attggatatt attcagcgac tagtaagcct atgtcatttc 1081 atccaccaca ttacttagca gccagacccg gtccgtttgg aatgcctggg atggtgccgc 1141 cgcatgttcc tcctcagatg ctcaacattc cgcagacctc tctgcaagca aagcccgtgg 1201 ccccacaggt gcccagccca gggggcgccc cgggccaggg tccatacccg tacagcctct 1261 ctgagccagc acctctcact ttggacacga gcgggaagaa tctgacggag cagaacagct 1321 acagcaacat tcctcacgaa gggaagcaca cgccgctgta tgagcggtcc tcgcccatca 1381 acccggccca gagcggcagc cccaaccacg tggattccgc ctacttccct ggctcttcta 1441 catcgtcatc ttccgacaac gacgagggca gcggaggggc gacaaaccat atcagcggga 1501 acaagattgg ctgggagaag acgggaagcc actcagagcc tcaggcacga ggagacccag 1561 gagaccaaac aaaggcagaa ggctcgtcca ctgcctcttc aggaagccaa ctagccgaag 1621 gcaagggaag ccagatgggc actgtccagc caatcccgtg cctcctgtcg atgcccacca 1681 ggaaccacat ggacatcacc acacctcccc tgccccccgt cgcacctgag gtgctgagag 1741 tggccgagca caggcacaag aaggggctga tgtaccccta catcttccat gtcctgacga 1801 agggtgaaat caaaattgct gtttctattg aagatgaagc caacaaggac ctgcctccgg 1861 ccgctctgct ctataggcca gttcgtcagt atgtttacgg agtcctgttt agtttggcgg 1921 aaagcagaaa gaaaactgag agacttgctt ttagaaagaa cagacttcca ccagaatttt 1981 caccagtgat cattaaagaa tgggcagctt acaaaggaaa gtctcctcaa accccggaac 2041 tggttgaagc tcttgccttc agggagtgga cctgccccaa cctgaagagg ctgtggttgg 2101 gtaaggcggt agaggacaag aaccgcagga tgagggcctt cctggcctgc atgaggtcgg 2161 acaccccagc catgctcaac cctgccaacg tgcccactca cctcatggtg ctctgctgcg 2221 tcttacggta catggtgcag tggccgggag cacgcatcct tcggcgtcag gagctagatg 2281 ccttcctggc tcaggcgctg tcccccaaac tctacgagcc tgatcagctc caggagctca 2341 agattgagaa cctagatccc cgaggaattc agctatcagc tctcttcatg agtggagtag 2401 acatggcctt gtttgcaaat gatgcatgcg gacagccaat cccctgggaa cactgttgtc 2461 cttggatgta ttttgatggg aagctcttcc aatccaaact cctcaaagcc agccgggaaa 2521 agaccccact cattgacctc tgtgatggtc aggctgatca ggctgccaag gtagagaaga 2581 tgcgccagag cgtcctcgag gggctcagct tctccaggca gagccacacg ctccctttcc 2641 cgccgccacc tgccctgccc ttctaccctg cctctgcgta cccccggcac tttgggcctg 2701 tcccaccctc tcagggcagg ggcagaggct ttgcaggcgt ctgtggcttt ggaggcccct 2761 atggggaaac ggtagcaaca ggcccttacc gtgccttccg tgtggcggca gcatcgggac 2821 actgcggagc cttctcaggc agtgacagca gcaggactag caagtcccag ggcggagtcc 2881 aacctatacc ttctcaggga ggcaaactag aaatagctgg cactgtggtt ggccattggg 2941 ctgggagcag gcggggccgt gggggccggg ggcctttccc cctgcaggtg gtttctgtcg 3001 gaggaccagc tagagggcgt ccaagaggag ttatttccac cccagtgatt agaacatttg 3061 gaagaggtgg aaggtactat ggcagaggtt acaaaaacca ggcagcaatt cagggcagac 3121 ctccTTATGC TGCTTCAGCA GAAGAAGTGG CCAAAGAACT TAAGTCAAAA TCTGGGGAAT 3181 CGAAGTCCTC TGCTATGTCT TCAGACGGGT CCCTGGCTGA AAACGGAGTG ATGGCCGAGG 3241 AGAAGCCGGC TCCCCAGATG AACGGGAGCA CGGGTGACGC CAGGGCCCCC AGCCACTCTG 3301 AAAGTGCCTT GAATAATGAC TCTAAAACGT GCAATACAAA TCCTCATTTA AATGCACTAA 3361 GTACAGACAG CGCTTGCCGC AGAGAAGCTG CTCTGGAGGC AGCTGTCTTA AATAAAGAAG 3421 AGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 3481 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 3541 GGCTTTATAT ATCTTGTGGA AAGGACGATT GCCACTAGAC TTATAACTTA TAACGCGTTA 3601 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt