Transcript: Mouse NM_019639.4

Mus musculus ubiquitin C (Ubc), mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Ubc (22190)
Length:
2424
CDS:
112..2316

Additional Resources:

NCBI RefSeq record:
NM_019639.4
NBCI Gene record:
Ubc (22190)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_019639.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000304872 TTACCACCAAGAAGGTCAAAC pLKO_005 2225 CDS 100% 10.800 8.640 N Ubc n/a
2 TRCN0000374421 AGGTCAAACAGGAAGACAGAC pLKO_005 2237 CDS 100% 4.050 2.835 N Ubc n/a
3 TRCN0000311144 AGACGTACCTTCCTCACCACA pLKO_005 2254 CDS 100% 2.640 1.848 N Ubc n/a
4 TRCN0000374489 CACTTAGTCCTTCGCCTCAGA pLKO_005 997 CDS 100% 2.640 1.848 N Ubc n/a
5 TRCN0000098659 TCAGACTACAACATCCAGAAA pLKO.1 280 CDS 100% 4.950 2.970 N Ubc n/a
6 TRCN0000098655 CCATAGAGAATGTCAAGGCAA pLKO.1 404 CDS 100% 2.640 1.584 N Ubc n/a
7 TRCN0000098656 CCATTGAGAATGTCAAGGCAA pLKO.1 176 CDS 100% 2.640 1.584 N Ubc n/a
8 TRCN0000098657 CCTGTCAGACTACAACATCCA pLKO.1 276 CDS 100% 2.640 1.584 N Ubc n/a
9 TRCN0000316517 CCTGTCAGACTACAACATCCA pLKO_005 276 CDS 100% 2.640 1.584 N Ubc n/a
10 TRCN0000098658 TGACACCATAGAGAATGTCAA pLKO.1 399 CDS 100% 0.495 0.297 N Ubc n/a
11 TRCN0000316601 TGACACCATAGAGAATGTCAA pLKO_005 399 CDS 100% 0.495 0.297 N Ubc n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_019639.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14873 pDONR223 70.1% 28.1% 31% None (many diffs) n/a
2 ccsbBroad304_14873 pLX_304 0% 28.1% 31% V5 (many diffs) n/a
3 ccsbBroadEn_14872 pDONR223 58.4% 27.9% 31% None (many diffs) n/a
4 ccsbBroad304_14872 pLX_304 0% 27.9% 31% V5 (many diffs) n/a
5 TRCN0000468005 TCGGGCCTGTCTTATCTTATATCC pLX_317 100% 9.3% 10.3% V5 (many diffs) n/a
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