Transcript: Mouse NM_019702.2

Mus musculus Hbs1-like (S. cerevisiae) (Hbs1l), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Hbs1l (56422)
Length:
2793
CDS:
156..2204

Additional Resources:

NCBI RefSeq record:
NM_019702.2
NBCI Gene record:
Hbs1l (56422)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_019702.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000250812 ATTGAAGTTCCGATCACTAAA pLKO_005 1917 CDS 100% 13.200 18.480 N Hbs1l n/a
2 TRCN0000250813 CTGTTATTAAGCGATTGATTA pLKO_005 1981 CDS 100% 13.200 18.480 N Hbs1l n/a
3 TRCN0000217852 GTTAGCTGCCAACAAGTATTA pLKO.1 2325 3UTR 100% 13.200 18.480 N Hbs1l n/a
4 TRCN0000258111 ACGTCGGAATGACGAAGTTTG pLKO_005 1129 CDS 100% 10.800 15.120 N Hbs1l n/a
5 TRCN0000202317 GCTCCTCAACTTAGTGGTCAT pLKO.1 926 CDS 100% 4.050 5.670 N Hbs1l n/a
6 TRCN0000191758 GCTGTTATTAAGCGATTGATT pLKO.1 1980 CDS 100% 5.625 4.500 N Hbs1l n/a
7 TRCN0000250811 TTAGCTGCCAACAAGTATTAA pLKO_005 2326 3UTR 100% 15.000 10.500 N Hbs1l n/a
8 TRCN0000250814 GGCGGTTTATGCTGCGTTATG pLKO_005 2137 CDS 100% 10.800 7.560 N Hbs1l n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_019702.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02521 pDONR223 100% 85.3% 88.6% None (many diffs) n/a
2 ccsbBroad304_02521 pLX_304 0% 85.3% 88.6% V5 (many diffs) n/a
3 TRCN0000476887 CTCATTAGAGTGAGAAGAGATGTA pLX_317 17.9% 85.3% 88.6% V5 (many diffs) n/a
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