Construct: ORF ccsbBroad304_02521

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009392.1_s304c1, BRDN0000406911
Derived from:
ccsbBroadEn_02521
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HBS1L (10767)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02521
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10767 HBS1L HBS1 like translational GTPase NM_006620.4 100% 100%
2 human 10767 HBS1L HBS1 like translational GTPase NM_001145158.2 93.8% 93.8% 107_108ins126
3 human 10767 HBS1L HBS1 like translational GTPase NM_001363686.2 76% 76% 0_1ins492
4 human 10767 HBS1L HBS1 like translational GTPase XR_002956256.1 68.8% (many diffs)
5 human 10767 HBS1L HBS1 like translational GTPase XR_245499.2 68.6% 1_135del;1932_1933ins101;2087_2743del
6 human 10767 HBS1L HBS1 like translational GTPase XR_002956257.1 49% (many diffs)
7 mouse 56422 Hbs1l Hbs1-like (S. cerevisiae) NM_019702.2 85.3% 88.6% (many diffs)
8 mouse 56422 Hbs1l Hbs1-like (S. cerevisiae) NM_001042593.1 84.9% 88.3% (many diffs)
9 mouse 56422 Hbs1l Hbs1-like (S. cerevisiae) XM_006512803.3 84.4% 87.8% (many diffs)
10 mouse 56422 Hbs1l Hbs1-like (S. cerevisiae) XM_006512804.2 65.1% 68.9% (many diffs)
11 mouse 56422 Hbs1l Hbs1-like (S. cerevisiae) XM_006512805.3 65.1% 68.9% (many diffs)
12 mouse 56422 Hbs1l Hbs1-like (S. cerevisiae) XR_380070.1 59.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2118
ORF length:
2052
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ccggcatcgg aatgttcgag gctataacta cgatgaagat tttgaagatg
121atgatctcta cggccagtct gtagaggatg attattgtat ttcgccgtca acagctgctc
181agtttattta ttcacggcgt gacaaacctt ccgttgagcc tgtggaagaa tatgattatg
241aagatctgaa agaatcttcc aattctgttt caaaccatca gctcagtgga tttgatcaag
301ctcgtcttta ttcatgcctt gatcacatga gagaggtact tggagatgct gtgccagatg
361aaatattaat tgaagcagtt ctgaagaaca agtttgatgt gcagaaggct ttgtcagggg
421ttctggaaca agatagagtg cagagtttga aggacaagaa tgaggcaaca gtatctacag
481gaaagatagc aaaaggaaaa ccagtagatt cccagacatc gcgaagtgaa tctgaaattg
541tgccaaaagt tgctaaaatg actgtatctg gaaagaagca aactatggga tttgaagtgc
601ctggagtatc ttctgaagaa aatggtcata gtttccacac acctcaaaaa ggaccgccca
661ttgaagatgc cattgcttct tccgatgttc ttgagactgc ttctaaatct gctaatccac
721cccacacgat tcaagcatca gaagagcaga gttcaacccc agcaccggtg aaaaagtctg
781gcaagctgag gcagcaaata gatgtgaagg cggaactgga gaagcggcaa ggagggaagc
841agctactcaa cttagtggtc attggtcatg ttgatgctgg gaaaagtact ctgatgggcc
901atatgcttta tcttctgggt aatataaaca aaagaactat gcataagtat gaacaggagt
961ctaaaaaggc tggcaaagct tcgtttgcat atgcatgggt cttggatgaa actggcgaag
1021aaagggaaag gggagtaacc atggatgttg gtatgacaaa gtttgaaacc acaaccaaag
1081ttattacatt aatggatgct ccaggccata aggacttcat tccaaatatg attacaggag
1141cagcccaggc ggatgtagct gttttagttg tagatgccag caggggagag tttgaagctg
1201gatttgagac tggaggacaa acacgagagc atggactctt ggtccgttct ctgggagtga
1261cgcagcttgc agttgcagtt aataaaatgg atcaggttaa ttggcaacaa gaaaggtttc
1321aagagattac tggaaaactt gggcactttc ttaagcaagc aggttttaag gagagtgatg
1381taggttttat tcctacaagt ggtctcagtg gtgaaaatct aatcacaaga tctcagtcaa
1441gtgaactcac aaaatggtat aaaggactat gtttattaga acaaattgat tcctttaagc
1501ctccccagcg atctattgac aaacctttta gattatgtgt gtccgatgtt ttcaaagatc
1561aaggatctgg attttgcata actggtaaaa tagaagctgg ttatatccaa actggtgacc
1621gactactggc aatgcctcct aatgaaactt gtaccgtgaa aggaatcact ctgcatgatg
1681aacctgtcga ctgggcggca gcaggcgatc atgttagtct tactttggtt gggatggata
1741tcatcaaaat caatgttggc tgcatatttt gtggccccaa agtacccatt aaagcttgca
1801ctcgtttcag agcccgaatc ctcatcttta atattgaaat tcctatcact aaaggatttc
1861ctgtgctgtt acactaccaa actgtcagtg aacccgccgt tattaaacga ttgattagtg
1921tcttaaacaa aagcacgggt gaagtcacaa agaaaaagcc taagtttttg actaaaggcc
1981agaatgcatt ggtagagcta cagacacaaa gaccaatagc tcttgagcta tataaagact
2041ttaaagagct ggggaggttc atgctacgtt acggtggttc tacaatagct gctggtgttg
2101tcactgagat aaaagaatgc ccaactttct tgtacaaagt ggttggtaag cctatcccta
2161accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
2221agtcgacaat caacctctgg atta

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