Construct: ORF ccsbBroad304_02521
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009392.1_s304c1, BRDN0000406911
- Derived from:
- ccsbBroadEn_02521
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HBS1L (10767)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02521
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10767 | HBS1L | HBS1 like translational GTPase | NM_006620.4 | 100% | 100% | |
| 2 | human | 10767 | HBS1L | HBS1 like translational GTPase | NM_001145158.2 | 93.8% | 93.8% | 107_108ins126 |
| 3 | human | 10767 | HBS1L | HBS1 like translational GTPase | NM_001363686.2 | 76% | 76% | 0_1ins492 |
| 4 | human | 10767 | HBS1L | HBS1 like translational GTPase | XR_002956256.1 | 68.8% | (many diffs) | |
| 5 | human | 10767 | HBS1L | HBS1 like translational GTPase | XR_245499.2 | 68.6% | 1_135del;1932_1933ins101;2087_2743del | |
| 6 | human | 10767 | HBS1L | HBS1 like translational GTPase | XR_002956257.1 | 49% | (many diffs) | |
| 7 | mouse | 56422 | Hbs1l | Hbs1-like (S. cerevisiae) | NM_019702.2 | 85.3% | 88.6% | (many diffs) |
| 8 | mouse | 56422 | Hbs1l | Hbs1-like (S. cerevisiae) | NM_001042593.1 | 84.9% | 88.3% | (many diffs) |
| 9 | mouse | 56422 | Hbs1l | Hbs1-like (S. cerevisiae) | XM_006512803.3 | 84.4% | 87.8% | (many diffs) |
| 10 | mouse | 56422 | Hbs1l | Hbs1-like (S. cerevisiae) | XM_006512804.2 | 65.1% | 68.9% | (many diffs) |
| 11 | mouse | 56422 | Hbs1l | Hbs1-like (S. cerevisiae) | XM_006512805.3 | 65.1% | 68.9% | (many diffs) |
| 12 | mouse | 56422 | Hbs1l | Hbs1-like (S. cerevisiae) | XR_380070.1 | 59.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2118
- ORF length:
- 2052
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ccggcatcgg aatgttcgag gctataacta cgatgaagat tttgaagatg 121 atgatctcta cggccagtct gtagaggatg attattgtat ttcgccgtca acagctgctc 181 agtttattta ttcacggcgt gacaaacctt ccgttgagcc tgtggaagaa tatgattatg 241 aagatctgaa agaatcttcc aattctgttt caaaccatca gctcagtgga tttgatcaag 301 ctcgtcttta ttcatgcctt gatcacatga gagaggtact tggagatgct gtgccagatg 361 aaatattaat tgaagcagtt ctgaagaaca agtttgatgt gcagaaggct ttgtcagggg 421 ttctggaaca agatagagtg cagagtttga aggacaagaa tgaggcaaca gtatctacag 481 gaaagatagc aaaaggaaaa ccagtagatt cccagacatc gcgaagtgaa tctgaaattg 541 tgccaaaagt tgctaaaatg actgtatctg gaaagaagca aactatggga tttgaagtgc 601 ctggagtatc ttctgaagaa aatggtcata gtttccacac acctcaaaaa ggaccgccca 661 ttgaagatgc cattgcttct tccgatgttc ttgagactgc ttctaaatct gctaatccac 721 cccacacgat tcaagcatca gaagagcaga gttcaacccc agcaccggtg aaaaagtctg 781 gcaagctgag gcagcaaata gatgtgaagg cggaactgga gaagcggcaa ggagggaagc 841 agctactcaa cttagtggtc attggtcatg ttgatgctgg gaaaagtact ctgatgggcc 901 atatgcttta tcttctgggt aatataaaca aaagaactat gcataagtat gaacaggagt 961 ctaaaaaggc tggcaaagct tcgtttgcat atgcatgggt cttggatgaa actggcgaag 1021 aaagggaaag gggagtaacc atggatgttg gtatgacaaa gtttgaaacc acaaccaaag 1081 ttattacatt aatggatgct ccaggccata aggacttcat tccaaatatg attacaggag 1141 cagcccaggc ggatgtagct gttttagttg tagatgccag caggggagag tttgaagctg 1201 gatttgagac tggaggacaa acacgagagc atggactctt ggtccgttct ctgggagtga 1261 cgcagcttgc agttgcagtt aataaaatgg atcaggttaa ttggcaacaa gaaaggtttc 1321 aagagattac tggaaaactt gggcactttc ttaagcaagc aggttttaag gagagtgatg 1381 taggttttat tcctacaagt ggtctcagtg gtgaaaatct aatcacaaga tctcagtcaa 1441 gtgaactcac aaaatggtat aaaggactat gtttattaga acaaattgat tcctttaagc 1501 ctccccagcg atctattgac aaacctttta gattatgtgt gtccgatgtt ttcaaagatc 1561 aaggatctgg attttgcata actggtaaaa tagaagctgg ttatatccaa actggtgacc 1621 gactactggc aatgcctcct aatgaaactt gtaccgtgaa aggaatcact ctgcatgatg 1681 aacctgtcga ctgggcggca gcaggcgatc atgttagtct tactttggtt gggatggata 1741 tcatcaaaat caatgttggc tgcatatttt gtggccccaa agtacccatt aaagcttgca 1801 ctcgtttcag agcccgaatc ctcatcttta atattgaaat tcctatcact aaaggatttc 1861 ctgtgctgtt acactaccaa actgtcagtg aacccgccgt tattaaacga ttgattagtg 1921 tcttaaacaa aagcacgggt gaagtcacaa agaaaaagcc taagtttttg actaaaggcc 1981 agaatgcatt ggtagagcta cagacacaaa gaccaatagc tcttgagcta tataaagact 2041 ttaaagagct ggggaggttc atgctacgtt acggtggttc tacaatagct gctggtgttg 2101 tcactgagat aaaagaatgc ccaactttct tgtacaaagt ggttggtaag cctatcccta 2161 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 2221 agtcgacaat caacctctgg atta