Transcript: Human NM_020825.4

Homo sapiens cramped chromatin regulator homolog 1 (CRAMP1), mRNA.

Source:
NCBI, updated 2019-09-15
Taxon:
Homo sapiens (human)
Gene:
CRAMP1 (57585)
Length:
7972
CDS:
300..4109

Additional Resources:

NCBI RefSeq record:
NM_020825.4
NBCI Gene record:
CRAMP1 (57585)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_020825.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000244951 CATCAGTATGCAGTCGGATTT pLKO_005 2888 CDS 100% 10.800 15.120 N CRAMP1 n/a
2 TRCN0000244949 TCACCAAGTACATCGACTTTG pLKO_005 967 CDS 100% 10.800 15.120 N CRAMP1 n/a
3 TRCN0000244952 TGGCCAGTGCTATCGACTTAG pLKO_005 3151 CDS 100% 10.800 15.120 N CRAMP1 n/a
4 TRCN0000183304 GATTACATTTCTCGGTTCAAT pLKO.1 3990 CDS 100% 5.625 7.875 N CRAMP1 n/a
5 TRCN0000128794 CTATCGACTTAGCAGCTACAA pLKO.1 3160 CDS 100% 4.950 6.930 N CRAMP1 n/a
6 TRCN0000195769 CCATCAGTATGCAGTCGGATT pLKO.1 2887 CDS 100% 4.050 5.670 N CRAMP1 n/a
7 TRCN0000128372 GCATTGATTACATTTCTCGGT pLKO.1 3985 CDS 100% 0.660 0.528 N CRAMP1 n/a
8 TRCN0000244953 GTGACCCTTAGGCGCTAATAT pLKO_005 4954 3UTR 100% 15.000 10.500 N CRAMP1 n/a
9 TRCN0000244950 GATGACAAGAATGCAACAAAG pLKO_005 1083 CDS 100% 10.800 7.560 N CRAMP1 n/a
10 TRCN0000257325 GATGACAAGAATGCAACAAAG pLKO_005 1083 CDS 100% 10.800 7.560 N Cramp1l n/a
11 TRCN0000129075 GCTCATCTGTTCTCTCCTTAT pLKO.1 3403 CDS 100% 10.800 7.560 N CRAMP1 n/a
12 TRCN0000130478 GTAAAGAAGCAGAGCTGACTT pLKO.1 2848 CDS 100% 4.950 2.970 N CRAMP1 n/a
13 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 7244 3UTR 100% 4.950 2.475 Y ERAP2 n/a
14 TRCN0000239422 ACCGCACCTGGCACAAGATTA pLKO_005 949 CDS 100% 13.200 7.920 N Cramp1l n/a
15 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 7245 3UTR 100% 13.200 6.600 Y LIAS n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_020825.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12369 pDONR223 100% 83.8% 83.7% None (many diffs) n/a
2 ccsbBroad304_12369 pLX_304 0% 83.8% 83.7% V5 (many diffs) n/a
3 TRCN0000476861 TGTGTAATGTGCCTTCTCCATTAA pLX_317 12.1% 83.8% 83.7% V5 (many diffs) n/a
Download CSV