Transcript: Human NM_022808.4

Homo sapiens small nuclear ribonucleoprotein polypeptide N (SNRPN), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-05-21
Taxon:
Homo sapiens (human)
Gene:
SNRPN (6638)
Length:
1823
CDS:
822..1544

Additional Resources:

NCBI RefSeq record:
NM_022808.4
NBCI Gene record:
SNRPN (6638)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_022808.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000414348 AGACACCAAGAGGTGGTTAAA pLKO_005 614 5UTR 100% 13.200 6.600 Y SNURF n/a
2 TRCN0000430814 TGCTGCAGCACATTGACTATA pLKO_005 847 CDS 100% 13.200 6.600 Y SNRPN n/a
3 TRCN0000075135 GAATCTTCATTGGCACCTTTA pLKO.1 895 CDS 100% 10.800 5.400 Y SNRPN n/a
4 TRCN0000413452 GAGTAGCGAGGAATCTGATTC pLKO_005 644 5UTR 100% 10.800 5.400 Y SNURF n/a
5 TRCN0000429886 TTGCTGCGACTGCCAGTATTG pLKO_005 1285 CDS 100% 10.800 5.400 Y SNRPN n/a
6 TRCN0000075137 CAGAAAGATCAAGCCAAAGAA pLKO.1 965 CDS 100% 5.625 2.813 Y SNRPN n/a
7 TRCN0000433444 GACCATCCCAGCAGGTAATGA pLKO_005 1228 CDS 100% 5.625 2.813 Y SNRPN n/a
8 TRCN0000123156 CAACCAAGAGTGTCAGTTGTA pLKO.1 519 5UTR 100% 4.950 2.475 Y SNURF n/a
9 TRCN0000075134 CCTCTGTGATTGTGATGAGTT pLKO.1 944 CDS 100% 4.950 2.475 Y SNRPN n/a
10 TRCN0000123158 CCTGAGACGAACTACAGAACA pLKO.1 444 5UTR 100% 4.950 2.475 Y SNURF n/a
11 TRCN0000075133 CGAATCTTCATTGGCACCTTT pLKO.1 894 CDS 100% 4.950 2.475 Y SNRPN n/a
12 TRCN0000123157 CGTTCTCAGCAGCAGCAAGTA pLKO.1 547 5UTR 100% 4.950 2.475 Y SNURF n/a
13 TRCN0000417615 GAAGCAACCAGAGCGTGAAGA pLKO_005 989 CDS 100% 4.950 2.475 Y SNRPN n/a
14 TRCN0000419956 GGTGGATTTCCAGGCTGAACT pLKO_005 573 5UTR 100% 4.950 2.475 Y SNURF n/a
15 TRCN0000111934 TGAGACACCAAGAGGTGGTTA pLKO.1 612 5UTR 100% 4.950 2.475 Y Snurf n/a
16 TRCN0000075136 GTTCAGAAAGATCAAGCCAAA pLKO.1 962 CDS 100% 4.050 2.025 Y SNRPN n/a
17 TRCN0000417704 GGTGGTTAAAGCCATATTGGA pLKO_005 625 5UTR 100% 3.000 1.500 Y SNURF n/a
18 TRCN0000123154 CGAACTACAGAACAGCACGTA pLKO.1 451 5UTR 100% 2.640 1.320 Y SNURF n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_022808.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01575 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01575 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000471606 ATAAAGGACCTTCCAAGGTTCAAG pLX_317 64.7% 99.8% 34.5% V5 (not translated due to prior stop codon) 255_256insC n/a
4 ccsbBroadEn_01568 pDONR223 100% 78.6% 88.3% None (many diffs) n/a
5 ccsbBroad304_01568 pLX_304 0% 78.6% 88.3% V5 (many diffs) n/a
6 TRCN0000473898 TGTCAGCATTCTACCTATAACCAG pLX_317 51% 78.6% 88.3% V5 (many diffs) n/a
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