Construct: ORF TRCN0000471606

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006951.1_s317c1
Derived from:
ccsbBroadEn_01575
DNA Barcode:
ATAAAGGACCTTCCAAGGTTCAAG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
SNRPN (6638)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471606
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6638 SNRPN small nuclear ribonucleopro... NM_001349454.1 99.8% 34.5% 255_256insC
2 human 6638 SNRPN small nuclear ribonucleopro... NM_001349455.1 99.8% 34.5% 255_256insC
3 human 6638 SNRPN small nuclear ribonucleopro... NM_001349456.1 99.8% 34.5% 255_256insC
4 human 6638 SNRPN small nuclear ribonucleopro... NM_001349457.1 99.8% 34.5% 255_256insC
5 human 6638 SNRPN small nuclear ribonucleopro... NM_001349458.1 99.8% 34.5% 255_256insC
6 human 6638 SNRPN small nuclear ribonucleopro... NM_001349459.1 99.8% 34.5% 255_256insC
7 human 6638 SNRPN small nuclear ribonucleopro... NM_001349460.1 99.8% 34.5% 255_256insC
8 human 6638 SNRPN small nuclear ribonucleopro... NM_001349461.1 99.8% 34.5% 255_256insC
9 human 6638 SNRPN small nuclear ribonucleopro... NM_001349462.1 99.8% 34.5% 255_256insC
10 human 6638 SNRPN small nuclear ribonucleopro... NM_001349463.1 99.8% 34.5% 255_256insC
11 human 6638 SNRPN small nuclear ribonucleopro... NM_001349464.1 99.8% 34.5% 255_256insC
12 human 6638 SNRPN small nuclear ribonucleopro... NM_001349465.1 99.8% 34.5% 255_256insC
13 human 6638 SNRPN small nuclear ribonucleopro... NM_003097.5 99.8% 34.5% 255_256insC
14 human 6638 SNRPN small nuclear ribonucleopro... NM_022805.4 99.8% 34.5% 255_256insC
15 human 6638 SNRPN small nuclear ribonucleopro... NM_022806.4 99.8% 34.5% 255_256insC
16 human 6638 SNRPN small nuclear ribonucleopro... NM_022807.4 99.8% 34.5% 255_256insC
17 human 6638 SNRPN small nuclear ribonucleopro... NM_022808.4 99.8% 34.5% 255_256insC
18 human 6628 SNRPB small nuclear ribonucleopro... NM_198216.2 81.9% 33.7% (many diffs)
19 human 6628 SNRPB small nuclear ribonucleopro... NM_003091.4 78.5% 34.9% (many diffs)
20 human 104472715 SNHG14 small nucleolar RNA host ge... NR_146177.1 3.7% 1_902del;1157_1158insC;1623_19263del
21 mouse 20646 Snrpn small nuclear ribonucleopro... NM_001082961.2 91.4% 34.5% (many diffs)
22 mouse 20646 Snrpn small nuclear ribonucleopro... NM_001082962.2 91.4% 34.5% (many diffs)
23 mouse 20646 Snrpn small nuclear ribonucleopro... NM_001349690.1 91.4% 34.5% (many diffs)
24 mouse 20646 Snrpn small nuclear ribonucleopro... NM_001349691.1 91.4% 34.5% (many diffs)
25 mouse 20646 Snrpn small nuclear ribonucleopro... NM_001349692.1 91.4% 34.5% (many diffs)
26 mouse 20646 Snrpn small nuclear ribonucleopro... NM_001349693.1 91.4% 34.5% (many diffs)
27 mouse 20646 Snrpn small nuclear ribonucleopro... NM_001349694.1 91.4% 34.5% (many diffs)
28 mouse 20646 Snrpn small nuclear ribonucleopro... NM_001349695.1 91.4% 34.5% (many diffs)
29 mouse 20646 Snrpn small nuclear ribonucleopro... NM_013670.4 91.4% 34.5% (many diffs)
30 mouse 20638 Snrpb small nuclear ribonucleopro... NM_009225.2 78.3% 34.9% (many diffs)
31 mouse 52480 Snhg14 small nucleolar RNA host ge... NR_146211.1 2.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
387
ORF length:
321
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac tgttggcaag agtagcaaga tgctgcagca cattgactat agaatgagat
121gtatcctgca agatggccga atcttcattg gcacctttaa ggcttttgac aagcatatga
181atttgatcct ctgtgattgt gatgagttca gaaagatcaa gccaaagaat gcgaagcaac
241cagagcgtga agaaaagcgg gttttgggtc tggtgttgct gcgtggggag aacttggtat
301ccatgactgt ggaggggCCA CCCCCCCAAA GATACTGGCA TTGCTCGGGT ACCACTTGCT
361GGAGCTGCTG GAGGCCCTGG GGTTGGTAGG GCAGCTGGTA GAGGAGTACC AGCTGGTGTG
421CCAATTCCCC AGGCCCCTGC TGGATTGGCA GGCCCTGTCC GAGGAGTTGG GGGACCATCC
481CAGCAGGTAA TGACTCCACA GGGAAGAGGC ACTGTAGCAG CTGCTGCTGT TGCTGCGACT
541GCCAGTATTG CTGGAGCCCC AACACAGTAC CCACCAGGAC GGGGCACTCC GCCCCCACCC
601GTCGGCAGAG CAACCCCACC TCCAGGCATT ATGGCTCCTC CACCTGGTAT GAGACCACCC
661ATGGGCCCAC CAATTGGGCT TCCCCCTGCT CGAGGGACGC CAATAGGCAT GCCGCCTCCG
721GGAATGAGAC CCCCTCCACC AGGCATTAGA GGTCCACCTC CCCCAGGAAT GCGTCCACCA
781AGACCTTACC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT
841CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT
901TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATAAAGGA CCTTCCAAGG TTCAAGACGC
961GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)