Construct: ORF TRCN0000491438
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001538.1_s317c1
- Derived from:
- ccsbBroadEn_06859
- DNA Barcode:
- GGGTTAACCCAGCTTGCTCCGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RFX2 (5990)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491438
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5990 | RFX2 | regulatory factor X2 | NM_000635.4 | 99.9% | 99.7% | 8A>N;1829G>A |
| 2 | human | 5990 | RFX2 | regulatory factor X2 | NM_134433.3 | 96.4% | 96.2% | 8A>N;522_523ins75;1754G>A |
| 3 | human | 5990 | RFX2 | regulatory factor X2 | XM_011528172.2 | 94.7% | 94.4% | (many diffs) |
| 4 | human | 5990 | RFX2 | regulatory factor X2 | XM_017027107.1 | 93.7% | 93.6% | 0_1ins135;1694G>A |
| 5 | human | 5990 | RFX2 | regulatory factor X2 | XR_001753732.2 | 73.6% | (many diffs) | |
| 6 | mouse | 19725 | Rfx2 | regulatory factor X, 2 (inf... | NM_027787.1 | 83.3% | 87.5% | (many diffs) |
| 7 | mouse | 19725 | Rfx2 | regulatory factor X, 2 (inf... | NM_009056.2 | 80.5% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2235
- ORF length:
- 2169
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ganttccgag ggtggagcgg attcgccagc gtccgtggct ctgcgtccct 121 cggcggcagc cccgcctgtg ccagcctccc cgcagagggt gttggtccag gcagccagct 181 ccaatcccaa aggggcccag atgcagccga tctccctccc cagagttcag caggtacccc 241 agcaggtgca gccggtgcag cacgtgtatc ctgcccaggt gcagtacgtg gaagggggag 301 acgccgtcta caccaatgga gccatacgaa cagcctacac ctacaacccc gagcctcaga 361 tgtacgcccc cagcagcacg gcttcttact tcgaggcccc aggcggtgcc caggtgaccg 421 tggcagcctc gtccccgcca gcggtcccct cccacagcat ggtgggcatc accatggatg 481 tcggggggag ccccatcgtc tccagcgcgg gagcctatct catccacggg gggatggaca 541 gcaccagaca ctccctggcc cacacctccc gctcatcgcc cgccacgctt gaaatggcga 601 ttgaaaacct ccaaaaaagc gaaggaatca catcacacaa aagcggttta ctcaacagcc 661 atctccagtg gctgttggat aattatgaaa cagcggaagg tgtgagtctc cccagaagtt 721 ctctttacaa ccactacctt cggcactgcc aggagcacaa gctagaccca gtgaacgccg 781 cctccttcgg gaaactgatc cgttctgtgt ttatggggct gagaacgcgg cggctgggca 841 ccaggggcaa ctcgaagtac cattactatg ggattcgtct gaagccggac tcaccactga 901 accggctgca ggaggacacg cagtacatgg ccatgcggca gcagcccatg caccagaagc 961 ccaggtaccg gccagcccag aagacggaca gcctcgggga cagcggctcc cacagcggcc 1021 tgcacagcac tccggaacag accatggccg tgcagagcca gcaccaccag cagtacatag 1081 atgtctccca cgtcttcccc gagttcccag cgcccgacct gggcagcttc ctgctgcagg 1141 acggcgtcac actgcacgac gtcaaggccc tgcagctggt gtacagacgg cactgcgagg 1201 caactgtaga tgtggtgatg aacctccagt tccactacat cgagaagctg tggctctcct 1261 tctggaactc taaggcctcc tccagcgacg gccccacctc tcttcctgcc agtgacgaag 1321 accccgaggg cgccgtcctg cccaaggaca agcttatctc cctgtgtcag tgcgacccca 1381 tcctcaggtg gatgaggagc tgcgaccaca tcctctacca ggcgctggtg gagattctca 1441 tccccgacgt gctgaggccg gtccccagta ccttgacaca ggccatccgt aactttgcca 1501 agagcttgga aggctggttg acaaatgcca tgagtgactt cccacaacag gtcatccaga 1561 ccaaggtggg cgtcgtcagt gccttcgccc agacgctgcg gcgctacacg tccctcaacc 1621 acctggcgca ggcggcccgg gcggtgctgc agaacacgtc ccagatcaac cagatgctca 1681 gcgacctcaa ccgcgtggac tttgccaacg tgcaggagca ggcctcgtgg gtgtgccagt 1741 gcgaggagag tgtggtgcag cggctggagc aggatttcaa gctgaccctg cagcagcaga 1801 gctccctgga ccagtgggcc agctggctgg acagtgtggt cacccaggtc ctgaagcagc 1861 atgccggcag ccccagcttc cccaaggccg cccagcagtt cttgctgaaa tggtcctttt 1921 acagctccat ggtgatccgg gacctgaccc tgcgcagcgc tgccagcttc ggctccttcc 1981 acctcatccg cctgctcTAC GACGAGTACA TGTTCTACCT GGTGGAGCAC CGCGTCGCGG 2041 AGGCCACCGG AGAGACGCCG ATCGCTGTGA TGGGAGAGTT CAACGATCTC GCCTCTCTGT 2101 CGCTGACGCT GCTCGACAAA GATGACATGG GCGATGAGCA GCGTGGCAGC GAGGCGGGCC 2161 CAGACGCCCG CAGCCTGGGT GAGCCCCTGG TAAAGCGGGA GCGCAGTGAC CCCAACCACT 2221 CCCTGCAGGG CATCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2281 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2341 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGGTTAACCC AGCTTGCTCC 2401 GTTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt