Transcript: Mouse NM_028149.1

Mus musculus F-box and leucine-rich repeat protein 20 (Fbxl20), mRNA.

Source:
NCBI, updated 2019-06-08
Taxon:
Mus musculus (mouse)
Gene:
Fbxl20 (72194)
Length:
8607
CDS:
260..1570

Additional Resources:

NCBI RefSeq record:
NM_028149.1
NBCI Gene record:
Fbxl20 (72194)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_028149.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000216606 GATCACGGATAGCACATTAAT pLKO.1 1192 CDS 100% 15.000 21.000 N Fbxl20 n/a
2 TRCN0000092363 CCCACCATGTTGAACTGAGTA pLKO.1 1582 3UTR 100% 4.950 6.930 N Fbxl20 n/a
3 TRCN0000092365 CGGCTTCAAGTGTTGAGTCTT pLKO.1 1235 CDS 100% 4.950 3.960 N Fbxl20 n/a
4 TRCN0000350173 CCATGTTGAACTGAGTATTTA pLKO_005 1586 3UTR 100% 15.000 10.500 N Fbxl20 n/a
5 TRCN0000217329 GAACTCCTGTTGAGGATATTT pLKO.1 350 CDS 100% 15.000 10.500 N Fbxl20 n/a
6 TRCN0000092367 GATGAGGCTGTAATCAATAAA pLKO.1 317 CDS 100% 15.000 10.500 N Fbxl20 n/a
7 TRCN0000317953 GATGAGGCTGTAATCAATAAA pLKO_005 317 CDS 100% 15.000 10.500 N Fbxl20 n/a
8 TRCN0000092364 GCCCTGAGCTAGTGACTTTAA pLKO.1 918 CDS 100% 13.200 9.240 N Fbxl20 n/a
9 TRCN0000318022 GCCCTGAGCTAGTGACTTTAA pLKO_005 918 CDS 100% 13.200 9.240 N Fbxl20 n/a
10 TRCN0000314214 TTGGCTTCCTGTACGTCAATA pLKO_005 707 CDS 100% 13.200 9.240 N Fbxl20 n/a
11 TRCN0000314157 TAGGAATATTGAAGTACTAAG pLKO_005 607 CDS 100% 10.800 7.560 N Fbxl20 n/a
12 TRCN0000192702 GCTCATAAGACTTCCTTTCCA pLKO.1 7215 3UTR 100% 3.000 2.100 N Fbxl20 n/a
13 TRCN0000092366 CCTTAGCAAGTTCTGCTCCAA pLKO.1 670 CDS 100% 2.640 1.848 N Fbxl20 n/a
14 TRCN0000217917 GGACCCATTTACCCAACATTA pLKO.1 1461 CDS 100% 13.200 7.920 N Fbxl20 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_028149.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04454 pDONR223 100% 93.1% 99.7% None (many diffs) n/a
2 ccsbBroad304_04454 pLX_304 0% 93.1% 99.7% V5 (many diffs) n/a
3 TRCN0000480672 ACGTCTTTACACTTGCCTAGATCA pLX_317 27.5% 93.1% 99.7% V5 (many diffs) n/a
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