Transcript: Human NM_031267.3

Homo sapiens cyclin dependent kinase 13 (CDK13), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
CDK13 (8621)
Length:
6805
CDS:
283..4641

Additional Resources:

NCBI RefSeq record:
NM_031267.3
NBCI Gene record:
CDK13 (8621)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148050 AGAATATGTGGGCCTCGCTA pXPR_003 TGG 2057 47% 4 1.2864 CDK13 CDK13 76626
2 BRDN0001147672 AGGTAACGGTGGTAATGTAG pXPR_003 AGG 1816 42% 2 0.5231 CDK13 CDK13 76625
3 BRDN0001146184 GGTTCCTTGTAGGCCGAAGG pXPR_003 CGG 870 20% 1 -0.1292 CDK13 CDK13 76627
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_031267.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000000704 CGATGTCTTCTTGCTGATTTA pLKO.1 2209 CDS 100% 13.200 18.480 N CDK13 n/a
2 TRCN0000352622 CGATGTCTTCTTGCTGATTTA pLKO_005 2209 CDS 100% 13.200 18.480 N CDK13 n/a
3 TRCN0000194677 CGATTGTACATCTTCACAAAT pLKO.1 4841 3UTR 100% 13.200 18.480 N CDK13 n/a
4 TRCN0000195638 CCACTAGAACGACGTAGTTTC pLKO.1 4186 CDS 100% 10.800 15.120 N CDK13 n/a
5 TRCN0000194804 CGACGTAGTTTCATTGGAAAT pLKO.1 4195 CDS 100% 10.800 8.640 N CDK13 n/a
6 TRCN0000342369 CGACGTAGTTTCATTGGAAAT pLKO_005 4195 CDS 100% 10.800 8.640 N CDK13 n/a
7 TRCN0000195065 CTGAGGAAGATCTAGATTATC pLKO.1 3914 CDS 100% 13.200 9.240 N CDK13 n/a
8 TRCN0000352631 CTGAGGAAGATCTAGATTATC pLKO_005 3914 CDS 100% 13.200 9.240 N CDK13 n/a
9 TRCN0000196896 GCTGATAGCTTACGAGGAAAT pLKO.1 2146 CDS 100% 10.800 7.560 N CDK13 n/a
10 TRCN0000352621 GCTGATAGCTTACGAGGAAAT pLKO_005 2146 CDS 100% 10.800 7.560 N CDK13 n/a
11 TRCN0000000702 GCTGCGCTAGACTTATTTGAT pLKO.1 3190 CDS 100% 5.625 3.938 N CDK13 n/a
12 TRCN0000000700 AGTGTTATTGTGAAAGGTGTA pLKO.1 5130 3UTR 100% 4.050 2.835 N CDK13 n/a
13 TRCN0000000701 CCTGAAGATAAAGAAGCTGAT pLKO.1 2131 CDS 100% 4.050 2.835 N CDK13 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_031267.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11288 pDONR223 100% 22% 21.4% None (many diffs) n/a
2 ccsbBroad304_11288 pLX_304 0% 22% 21.4% V5 (many diffs) n/a
3 TRCN0000470462 CGCGCTGGCGATCTTAACCCATCA pLX_317 41.9% 22% 21.4% V5 (many diffs) n/a
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