Construct: ORF TRCN0000470462
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012839.1_s317c1
- Derived from:
- ccsbBroadEn_11288
- DNA Barcode:
- CGCGCTGGCGATCTTAACCCATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDK13 (8621)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470462
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8621 | CDK13 | cyclin dependent kinase 13 | XM_011515597.3 | 29.4% | 27.1% | (many diffs) |
| 2 | human | 8621 | CDK13 | cyclin dependent kinase 13 | NM_031267.3 | 22% | 21.4% | (many diffs) |
| 3 | human | 8621 | CDK13 | cyclin dependent kinase 13 | XM_017012751.2 | 21.6% | 20.9% | (many diffs) |
| 4 | human | 8621 | CDK13 | cyclin dependent kinase 13 | NM_003718.5 | 21.1% | 20.5% | (many diffs) |
| 5 | human | 8621 | CDK13 | cyclin dependent kinase 13 | XM_017012750.2 | 20.7% | 20.1% | (many diffs) |
| 6 | mouse | 69562 | Cdk13 | cyclin-dependent kinase 13 | XM_011244344.3 | 32.8% | 33.6% | (many diffs) |
| 7 | mouse | 69562 | Cdk13 | cyclin-dependent kinase 13 | XM_017315593.2 | 31.6% | 33.1% | (many diffs) |
| 8 | mouse | 69562 | Cdk13 | cyclin-dependent kinase 13 | NM_027118.1 | 20.6% | 21.2% | (many diffs) |
| 9 | mouse | 69562 | Cdk13 | cyclin-dependent kinase 13 | XM_006516767.4 | 20.4% | 21% | (many diffs) |
| 10 | mouse | 69562 | Cdk13 | cyclin-dependent kinase 13 | NM_001081058.2 | 19.8% | 20.3% | (many diffs) |
| 11 | mouse | 69562 | Cdk13 | cyclin-dependent kinase 13 | XM_006516766.4 | 19.6% | 20.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1038
- ORF length:
- 972
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gcctgaagat aaagaagctg atagcttacg aggaaatatt tcagtaaaag 121 cagttaaaaa agaagtagaa aagaaactcc gatgtcttct tgctgattta ccgctgcccc 181 ctgagctacc aggaggagat gatctttcaa agagtccaga ggaaaagaaa acagcaacac 241 agttacatag taaaaggagg cctaaaatat gtgggcctcg ctatggtgaa accaaagaaa 301 aagatattga ctggggaaaa cgctgcgtgg ataaatttga tatcatcgga attattggag 361 aaggtactta cggacaagtt tacaaagcca gggataaaga cactggagaa atggtagcct 421 taaaaaaagt acgtctggat aatgaaaagg aaggctttcc aattacagca attcgagaaa 481 ttaaaattct ccggcagctt acccatcaga gtattatcaa tatgaaggaa atagtgactg 541 ataaagaaga tgctttggat ttcaagaagg acaaaggtgc attttatctg gtgtttgaat 601 atatggacca tgatctgatg ggactactgg aaTCAGGCTT GGTTCATTTT AATGAAAATC 661 ACATAAAGTC ATTTATGAGA CAGCTCATGG AGGGTCTGGA TTATTGTCAT AAGAAGAACT 721 TTTTGCATAG AGATATTAAA TGTTCCAATA TCCTTCTAAA TAATAGAGGG CAGATAAAAC 781 TTGCAGACTT TGGACTTGCT CGATTGTATA GCTCAGAAGA AAGTCGGCCG TATACTAACA 841 AGGTAATTAC TTTATGGTAC CGTCCACCTG AACTGCTACT GGGAGAAGAA CGATACACAC 901 CAGCCATTGA TGTATGGAGC TGTGGCTGTA TCCTTGGCGA ACTCTTCACT AAAAAACCTA 961 TATTTCAAGC AAATCAGGAA CTTGCACAAC TAGAATTAAT AAGACATGAA GAAAATGAAG 1021 TTTCTGATAA ACAAATCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1081 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1141 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACGCGCTG GCGATCTTAA 1201 CCCATCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt