Transcript: Mouse NM_144794.2

Mus musculus transmembrane protein 63a (Tmem63a), mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Tmem63a (208795)
Length:
3239
CDS:
57..2471

Additional Resources:

NCBI RefSeq record:
NM_144794.2
NBCI Gene record:
Tmem63a (208795)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_144794.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000304794 ACGTCCCAATGACTCCTATTG pLKO_005 131 CDS 100% 10.800 15.120 N Tmem63a n/a
2 TRCN0000304851 CCGAGAAGAGCCTTACCTATT pLKO_005 889 CDS 100% 10.800 8.640 N Tmem63a n/a
3 TRCN0000126436 GCTAGTGACAAAGGAAGCGAA pLKO.1 2253 CDS 100% 2.640 2.112 N Tmem63a n/a
4 TRCN0000349169 GCTAGTGACAAAGGAAGCGAA pLKO_005 2253 CDS 100% 2.640 2.112 N Tmem63a n/a
5 TRCN0000304852 GCCTTTAGGGAGATCATATTT pLKO_005 2841 3UTR 100% 15.000 10.500 N Tmem63a n/a
6 TRCN0000126434 CCGTATTGTGAGAAGAGGAAA pLKO.1 2577 3UTR 100% 4.950 3.465 N Tmem63a n/a
7 TRCN0000126435 CCTCTATACCTTCCGCATGAT pLKO.1 1790 CDS 100% 4.950 3.465 N Tmem63a n/a
8 TRCN0000316315 CCTCTATACCTTCCGCATGAT pLKO_005 1790 CDS 100% 4.950 3.465 N Tmem63a n/a
9 TRCN0000126437 CTCTTAGATGTCAGTTGCTTT pLKO.1 219 CDS 100% 4.950 3.465 N Tmem63a n/a
10 TRCN0000126438 CTGTTTCTAATCCTGGTGTTT pLKO.1 240 CDS 100% 4.950 3.465 N Tmem63a n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_144794.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02232 pDONR223 100% 87.2% 89.7% None (many diffs) n/a
2 ccsbBroad304_02232 pLX_304 0% 87.2% 89.7% V5 (many diffs) n/a
3 TRCN0000472089 CCCTAGCCAGCCTCGAGAGCTGGG pLX_317 15.3% 87.2% 89.7% V5 (many diffs) n/a
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