Transcript: Human NM_145241.5

Homo sapiens WD repeat domain 31 (WDR31), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
WDR31 (114987)
Length:
4868
CDS:
241..1341

Additional Resources:

NCBI RefSeq record:
NM_145241.5
NBCI Gene record:
WDR31 (114987)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_145241.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000243984 AGACAGTTGTGGCCTATAATT pLKO_005 485 CDS 100% 15.000 21.000 N WDR31 n/a
2 TRCN0000257208 ATACGGGCCTGATGAAATTAT pLKO_005 351 CDS 100% 15.000 21.000 N WDR31 n/a
3 TRCN0000146552 CAGAAGGTTTCGTTTAGGTTT pLKO.1 280 CDS 100% 4.950 3.960 N WDR31 n/a
4 TRCN0000243983 ATCACCAAGGTAGCCTGTATT pLKO_005 553 CDS 100% 13.200 9.240 N WDR31 n/a
5 TRCN0000243985 TCAGTGTTGTAAGTCCAATAA pLKO_005 1475 3UTR 100% 13.200 9.240 N WDR31 n/a
6 TRCN0000243986 CTCCACAGAAGGTTTCGTTTA pLKO_005 275 CDS 100% 10.800 7.560 N WDR31 n/a
7 TRCN0000150029 CATGATTGCAAGGTGAAGATT pLKO.1 1141 CDS 100% 5.625 3.938 N WDR31 n/a
8 TRCN0000179567 GAACATGAGATCACCAAGGTA pLKO.1 544 CDS 100% 3.000 2.100 N WDR31 n/a
9 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 3598 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_145241.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04673 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04673 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000470624 GCAATCACTTGGACAAACTCACCC pLX_317 41.8% 100% 100% V5 n/a
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