Transcript: Human NM_153767.3

Homo sapiens potassium inwardly rectifying channel subfamily J member 1 (KCNJ1), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
KCNJ1 (3758)
Length:
2760
CDS:
526..1644

Additional Resources:

NCBI RefSeq record:
NM_153767.3
NBCI Gene record:
KCNJ1 (3758)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_153767.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000005597 CAGTCTATACTTGGAGTTATA pLKO.1 955 CDS 100% 13.200 18.480 N KCNJ1 n/a
2 TRCN0000068527 CCGAGTGGATTTCCATAACTT pLKO.1 1479 CDS 100% 5.625 7.875 N Kcnj1 n/a
3 TRCN0000005596 CGAGTGGATTTCCATAACTTT pLKO.1 1480 CDS 100% 5.625 7.875 N KCNJ1 n/a
4 TRCN0000429463 GAACGTGCAATGCATTTATAT pLKO_005 1833 3UTR 100% 15.000 10.500 N KCNJ1 n/a
5 TRCN0000431927 TATGAAGCAGTCAACAATTTA pLKO_005 1704 3UTR 100% 15.000 10.500 N KCNJ1 n/a
6 TRCN0000005593 CGCTTACACAAAGAACAATTT pLKO.1 2151 3UTR 100% 13.200 9.240 N KCNJ1 n/a
7 TRCN0000429470 ACCAGATCAATATCAACTTTG pLKO_005 1202 CDS 100% 10.800 7.560 N KCNJ1 n/a
8 TRCN0000005594 CCCAACTTCATCTTGTCAGAA pLKO.1 1594 CDS 100% 4.950 3.465 N KCNJ1 n/a
9 TRCN0000005595 GCAGTAGCGTACATTCACAAA pLKO.1 769 CDS 100% 4.950 3.465 N KCNJ1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_153767.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00894 pDONR223 100% 95.1% 95.1% None 0_1ins57 n/a
2 ccsbBroad304_00894 pLX_304 0% 95.1% 95.1% V5 0_1ins57 n/a
3 TRCN0000479968 CAGCTTGAACAGCCCGGGCCTTGT pLX_317 26% 95.1% 95.1% V5 0_1ins57 n/a
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