Transcript: Mouse NM_172860.2

Mus musculus core-binding factor, runt domain, alpha subunit 2, translocated to, 2 (human) (Cbfa2t2), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Cbfa2t2 (12396)
Length:
6100
CDS:
120..1904

Additional Resources:

NCBI RefSeq record:
NM_172860.2
NBCI Gene record:
Cbfa2t2 (12396)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_172860.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000313730 TTGATGCCAACGGGCTCTAAG pLKO_005 1885 CDS 100% 10.800 15.120 N Cbfa2t2 n/a
2 TRCN0000095616 GCACCACAATCTTAGTCTCAA pLKO.1 1052 CDS 100% 4.950 3.960 N Cbfa2t2 n/a
3 TRCN0000095618 GCACTATACACTAGAAGACAT pLKO.1 968 CDS 100% 4.950 3.960 N Cbfa2t2 n/a
4 TRCN0000317316 GCACTATACACTAGAAGACAT pLKO_005 968 CDS 100% 4.950 3.960 N Cbfa2t2 n/a
5 TRCN0000313729 ACCGTGAAGAACTCAACTATT pLKO_005 1228 CDS 100% 13.200 9.240 N Cbfa2t2 n/a
6 TRCN0000313728 CACCAGTGAGCTGGCTATTAG pLKO_005 1955 3UTR 100% 13.200 9.240 N Cbfa2t2 n/a
7 TRCN0000095614 GCTGTTAAGATGCTCAGAAAT pLKO.1 2417 3UTR 100% 13.200 9.240 N Cbfa2t2 n/a
8 TRCN0000095615 CGTCCATTTGTGATTCCATTT pLKO.1 603 CDS 100% 10.800 7.560 N Cbfa2t2 n/a
9 TRCN0000095617 GCTCGAATGGAGCAGACCATA pLKO.1 1518 CDS 100% 4.950 3.465 N Cbfa2t2 n/a
10 TRCN0000317317 GCTCGAATGGAGCAGACCATA pLKO_005 1518 CDS 100% 4.950 3.465 N Cbfa2t2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_172860.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11342 pDONR223 100% 86.5% 91.8% None (many diffs) n/a
2 ccsbBroad304_11342 pLX_304 0% 86.5% 91.8% V5 (many diffs) n/a
3 TRCN0000467640 GGCTCATATTGGCAGGAGAAGAAG pLX_317 23.9% 86.5% 91.8% V5 (many diffs) n/a
4 ccsbBroadEn_11343 pDONR223 100% 35.7% 36.6% None (many diffs) n/a
5 ccsbBroad304_11343 pLX_304 0% 35.7% 36.6% V5 (many diffs) n/a
6 TRCN0000467899 TTTTCATGACACTCACTGATGGGC pLX_317 32% 35.7% 36.6% V5 (many diffs) n/a
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