Construct: ORF ccsbBroad304_11342
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000469.1_s304c1, BRDN0000409581
- Derived from:
- ccsbBroadEn_11342
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CBFA2T2 (9139)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11342
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9139 | CBFA2T2 | CBFA2/RUNX1 partner transcr... | XM_011529101.2 | 99.9% | 100% | 870C>T |
| 2 | human | 9139 | CBFA2T2 | CBFA2/RUNX1 partner transcr... | NM_001032999.3 | 96.5% | 95.2% | (many diffs) |
| 3 | human | 9139 | CBFA2T2 | CBFA2/RUNX1 partner transcr... | XM_017028122.2 | 96.4% | 95.1% | (many diffs) |
| 4 | human | 9139 | CBFA2T2 | CBFA2/RUNX1 partner transcr... | NM_005093.3 | 96.1% | 95.7% | (many diffs) |
| 5 | human | 9139 | CBFA2T2 | CBFA2/RUNX1 partner transcr... | NM_001039709.1 | 93.5% | 93.6% | 0_1ins117;753C>T |
| 6 | human | 9139 | CBFA2T2 | CBFA2/RUNX1 partner transcr... | XM_011529103.2 | 93.5% | 93.6% | 0_1ins117;753C>T |
| 7 | human | 9139 | CBFA2T2 | CBFA2/RUNX1 partner transcr... | XM_017028121.1 | 93.5% | 93.6% | 0_1ins117;753C>T |
| 8 | human | 9139 | CBFA2T2 | CBFA2/RUNX1 partner transcr... | XM_011529102.2 | 93.4% | 93.4% | 0_1ins117;753C>T;1235_1236insAGA |
| 9 | human | 9139 | CBFA2T2 | CBFA2/RUNX1 partner transcr... | XM_017028123.1 | 72.8% | 67.6% | (many diffs) |
| 10 | human | 9139 | CBFA2T2 | CBFA2/RUNX1 partner transcr... | XM_011529107.2 | 60.8% | 59.7% | (many diffs) |
| 11 | human | 9139 | CBFA2T2 | CBFA2/RUNX1 partner transcr... | XM_017028124.2 | 60.8% | 59.7% | (many diffs) |
| 12 | human | 9139 | CBFA2T2 | CBFA2/RUNX1 partner transcr... | XM_017028125.1 | 60.6% | 59.6% | (many diffs) |
| 13 | mouse | 12396 | Cbfa2t2 | core-binding factor, runt d... | NM_172860.2 | 86.5% | 91.8% | (many diffs) |
| 14 | mouse | 12396 | Cbfa2t2 | core-binding factor, runt d... | NM_009823.1 | 86.3% | 91.6% | (many diffs) |
| 15 | mouse | 12396 | Cbfa2t2 | core-binding factor, runt d... | XM_006498640.2 | 83.7% | 90.3% | (many diffs) |
| 16 | mouse | 12396 | Cbfa2t2 | core-binding factor, runt d... | XM_006498641.1 | 78.1% | 84.6% | (many diffs) |
| 17 | mouse | 12396 | Cbfa2t2 | core-binding factor, runt d... | XM_006498642.2 | 61.2% | 64.8% | (many diffs) |
| 18 | mouse | 12396 | Cbfa2t2 | core-binding factor, runt d... | NM_001285446.1 | 54.6% | 58.4% | (many diffs) |
| 19 | mouse | 12396 | Cbfa2t2 | core-binding factor, runt d... | XM_011239268.2 | 47.3% | 50.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1908
- ORF length:
- 1842
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg gtttcaccat gttggccagg ctcgtcttga actcctgacc tcaggtgatc 121 tgcctgcatt ggcctcccaa cgtgctggga ttacagttgg tcctgagaaa agggtgccag 181 cgatgcctgg atcgcctgtg gaagtgaaga tacagtccag atcctcacct cccaccatgc 241 cacccctccc accaataaat cctggaggac cgaggccagt gtccttcact cctactgcat 301 taagcaatgg catcaaccat tctcctccta ccctgaatgg tgccccatca ccgccacaga 361 gattcagcaa tggtcctgcc tcctccacat catctgcact cacaaatcag caattgccag 421 ccacttgtgg tgctcgacaa ctcagcaagt tgaaacgctt tcttaccact ctgcaacagt 481 ttggcaatga catctcccct gagattgggg agaaggtgcg gactcttgtt cttgcactgg 541 tgaactcaac agtgacaatt gaggaattcc actgtaagct ccaagaagcc acaaactttc 601 cccttcgtcc ttttgtgatt ccatttctca aggccaacct gcccctgctg cagcgggaac 661 tgctgcactg cgctcgggcg gccaagcaga ccccatccca gtacctggct cagcacgaac 721 accttctgct caacacaagc attgcatcgc ctgctgactc gtcagagttg ctcatggagg 781 tgcacggaaa tgggaagagg cccagtccag agaggagaga agagaatagt tttgatagag 841 acacaattgc tcctgagcct cctgccaaga gagtatgtac catcagccct gctcctcggc 901 acagtcctgc tctcactgtg cccctcatga atcctggggg ccaattccat cctacccctc 961 cacctcttca gcattacacc ttagaggata ttgcaacttc tcacctgtat cgggaaccca 1021 acaagatgct agagcatcga gaagttcgtg atagacacca cagtcttggt ctaaatggag 1081 gctatcaaga tgagttggta gatcatcgtt tgacagaaag ggaatgggct gatgaatgga 1141 aacatcttga ccatgcgctg aattgcatta tggaaatggt agagaaaaca aggcgctcta 1201 tggcagttct gcggcgctgt caggaatcag atcgtgaaga actcaactac tggaaaagac 1261 ggtacaatga aaacacagag ctgaggaaaa cggggaccga gttggtctcc aggcagcaca 1321 gccctgggag tgcagattct ctcagcaatg attctcagag agagttcaac agcaggccag 1381 gtacaggata cgtacctgtg gagttttgga aaaaaacaga agaagctgtg aataaggtga 1441 aaattcaggc catgtcagaa gtacagaagg ccgtcgctga ggcagagcag aaagcctttg 1501 aagtgattgc aacagagaga gcacgaatgg agcaaaccat agcggatgtc aagcggcagg 1561 ccgcagagga tgctttcctc gtcatcaatg agcaagagga gtccacggag aactgctgga 1621 actgtggccg caaagccagc gagacatgca gtggctgcaa tatcgcgcga tactgtggct 1681 ctttctgcca gcacaaggac tgggagcggc accaccgcct ctgtggtcag aacctgcatg 1741 gccagagccc ccacggccag ggccggccgc tgcttcctgt aggcaggggc tcctctgcca 1801 ggtccgccga ctgcagcgtg cccagcccag ccctcgacaa gacctcggca accacatcgc 1861 gttcctcaac acctgcttct gtgacagcta tcgacaccaa cggactctgc ccaactttct 1921 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1981 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta