Transcript: Human NM_181524.2

Homo sapiens phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-27
Taxon:
Homo sapiens (human)
Gene:
PIK3R1 (5295)
Length:
5712
CDS:
218..1492

Additional Resources:

NCBI RefSeq record:
NM_181524.2
NBCI Gene record:
PIK3R1 (5295)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145056 GTGATTATACTCTTACACTA pXPR_003 AGG 213 17% 3 0.7874 PIK3R1 PIK3R1 77091
2 BRDN0001148578 CCTACTACTGTAGCCAACAA pXPR_003 CGG 56 4% 2 0.1787 PIK3R1 PIK3R1 77092
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_181524.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000039903 GCGCTATGCAATTCTTAATTT pLKO.1 2293 3UTR 100% 15.000 21.000 N PIK3R1 n/a
2 TRCN0000416842 AGTGGTGGACGGCGAAGTAAA pLKO_005 1300 CDS 100% 13.200 18.480 N PIK3R1 n/a
3 TRCN0000413602 TTCATCGAGATGGGAAATATG pLKO_005 468 CDS 100% 13.200 18.480 N PIK3R1 n/a
4 TRCN0000033288 GCGGTACAGCAAAGAATACAT pLKO.1 823 CDS 100% 5.625 7.875 N PIK3R1 n/a
5 TRCN0000200002 CCGAGCCCTATAACTTGTACA pLKO.1 1362 CDS 100% 4.950 6.930 N PIK3R1 n/a
6 TRCN0000199643 GCCCTCAAACTTGACTCTACT pLKO.1 3729 3UTR 100% 4.950 6.930 N PIK3R1 n/a
7 TRCN0000039563 TACTGTAGCCAACAACGGTAT pLKO.1 262 CDS 100% 4.050 5.670 N PIK3R1 n/a
8 TRCN0000199461 GCAGAGGCACTCCTGATATAT pLKO.1 4291 3UTR 100% 15.000 12.000 N PIK3R1 n/a
9 TRCN0000432098 GGCAGCTGAGTATCGAGAAAT pLKO_005 973 CDS 100% 13.200 10.560 N PIK3R1 n/a
10 TRCN0000196285 GCATGGTGATTATACTCTTAC pLKO.1 409 CDS 100% 10.800 8.640 N PIK3R1 n/a
11 TRCN0000417698 ACATCGCACTGTGGATTATTT pLKO_005 1971 3UTR 100% 15.000 10.500 N PIK3R1 n/a
12 TRCN0000413458 AGTCGAGAATATGATAGATTA pLKO_005 695 CDS 100% 13.200 9.240 N PIK3R1 n/a
13 TRCN0000430557 TAACCATGGTGCTTGTTAATG pLKO_005 1817 3UTR 100% 13.200 9.240 N PIK3R1 n/a
14 TRCN0000430938 TACTTTATCCAGTATCCAAAT pLKO_005 588 CDS 100% 10.800 7.560 N PIK3R1 n/a
15 TRCN0000039904 CGAGGGAAGAAGTGAATGAAA pLKO.1 333 CDS 100% 5.625 3.938 N PIK3R1 n/a
16 TRCN0000039906 CGGTACAGCAAAGAATACATA pLKO.1 824 CDS 100% 5.625 3.938 N PIK3R1 n/a
17 TRCN0000033285 CCCATCATGATGAGAAGACAT pLKO.1 1167 CDS 100% 4.950 3.465 N PIK3R1 n/a
18 TRCN0000033287 CCTCAATGTCACACTAGCCTA pLKO.1 1444 CDS 100% 2.640 1.848 N PIK3R1 n/a
19 TRCN0000033284 CCTTCAGTTCTGTGGTTGAAT pLKO.1 507 CDS 100% 5.625 3.375 N PIK3R1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_181524.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06728 pDONR223 100% 92.7% 92% None (many diffs) n/a
2 ccsbBroad304_06728 pLX_304 47% 92.7% 92% V5 (many diffs) n/a
3 TRCN0000466732 GGCTCTGGTGCGGACAGCAGAAGC pLX_317 34.3% 92.7% 92% V5 (many diffs) n/a
4 ccsbBroadEn_14763 pDONR223 0% 92.7% 92% None (many diffs) n/a
5 ccsbBroad304_14763 pLX_304 49.6% 92.7% 92% V5 (many diffs) n/a
6 TRCN0000470397 TTCACCGGAGCCTGAGATAGGCTA pLX_317 32.4% 92.7% 92% V5 (many diffs) n/a
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