Construct: ORF TRCN0000470397
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008076.4_s317c1
- Derived from:
- ccsbBroadEn_14763
- DNA Barcode:
- TTCACCGGAGCCTGAGATAGGCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIK3R1 (5295)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470397
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5295 | PIK3R1 | phosphoinositide-3-kinase r... | NM_181504.4 | 99.9% | 99.7% | 541G>A |
| 2 | human | 5295 | PIK3R1 | phosphoinositide-3-kinase r... | NM_181524.2 | 92.7% | 92% | (many diffs) |
| 3 | human | 5295 | PIK3R1 | phosphoinositide-3-kinase r... | NM_001242466.2 | 79.4% | 79.2% | 0_1ins279;262G>A |
| 4 | human | 5295 | PIK3R1 | phosphoinositide-3-kinase r... | XM_011543493.3 | 71.4% | 66.4% | (many diffs) |
| 5 | human | 5295 | PIK3R1 | phosphoinositide-3-kinase r... | XM_017009586.1 | 69.4% | 64.6% | (many diffs) |
| 6 | human | 5295 | PIK3R1 | phosphoinositide-3-kinase r... | NM_181523.3 | 60.7% | 56.7% | (many diffs) |
| 7 | human | 5295 | PIK3R1 | phosphoinositide-3-kinase r... | XM_005248542.3 | 60.7% | 56.7% | (many diffs) |
| 8 | human | 5295 | PIK3R1 | phosphoinositide-3-kinase r... | XM_017009585.2 | 60.7% | 56.7% | (many diffs) |
| 9 | mouse | 18708 | Pik3r1 | phosphoinositide-3-kinase r... | NM_001024955.2 | 89.9% | 98.2% | (many diffs) |
| 10 | mouse | 18708 | Pik3r1 | phosphoinositide-3-kinase r... | XM_006517569.3 | 83.5% | 91.4% | (many diffs) |
| 11 | mouse | 18708 | Pik3r1 | phosphoinositide-3-kinase r... | XM_006517570.3 | 79.9% | 88.1% | (many diffs) |
| 12 | mouse | 18708 | Pik3r1 | phosphoinositide-3-kinase r... | NM_001077495.2 | 54.8% | 56.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1428
- ORF length:
- 1362
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta caatactgtt tggaatatgg aagacctgga tttagaatat gccaagacag 121 atataaattg tggcacagac ttgatgtttt atatagaaat ggacccacca gcactgcctc 181 ctaaaccacc aaaacctact actgtagcca acaacggtat gaataacaat atgtccttac 241 aagatgctga atggtactgg ggagatatct cgagggaaga agtgaatgaa aaacttcgag 301 atacagcaga cgggaccttt ttggtacgag atgcgtctac taaaatgcat ggtgattata 361 ctcttacact aaggaaaggg ggaaataaca aattaatcaa aatatttcat cgagatggga 421 aatatggctt ctctgaccca ttaaccttca gttctgtggt tgaattaata aaccactacc 481 ggaatgaatc tctagctcag tataatccca aattggatgt gaaattactt tatccagtat 541 ccaaatacca acaggatcaa gttgtcaaag aagataatat tgaagctgta gggaaaaaat 601 tacataaata taacactcag tttcaagaaa aaagtcgaga atatgataga ttatatgaag 661 aatatacccg cacatcccag gaaatccaaa tgaaaaggac agctattgaa gcatttaatg 721 aaaccataaa aatatttgaa gaacagtgcc agacccaaga gcggtacagc aaagaataca 781 tagaaaagtt taaacgtgaa ggcaatgaga aagaaataca aaggattatg cataattatg 841 ataagttgaa gtctcgaatc agtgaaatta ttgacagtag aagaagattg gaagaagact 901 tgaagaagca ggcagctgag tatcgagaaa ttgacaaacg tatgaacagc attaaaccag 961 accttatcca gctgagaaag acgagagacC AATACTTGAT GTGGTTGACT CAAAAAGGTG 1021 TTCGGCAAAA GAAGTTGAAC GAGTGGTTGG GCAATGAAAA CACTGAAGAC CAATATTCAC 1081 TGGTGGAAGA TGATGAAGAT TTGCCCCATC ATGATGAGAA GACATGGAAT GTTGGAAGCA 1141 GCAACCGAAA CAAAGCTGAA AACCTGTTGC GAGGGAAGCG AGATGGCACT TTTCTTGTCC 1201 GGGAGAGCAG TAAACAGGGC TGCTATGCCT GCTCTGTAGT GGTGGACGGC GAAGTAAAGC 1261 ATTGTGTCAT AAACAAAACA GCAACTGGCT ATGGCTTTGC CGAGCCCTAT AACTTGTACA 1321 GCTCTCTGAA AGAACTGGTG CTACATTACC AACACACCTC CCTTGTGCAG CACAACGACT 1381 CCCTCAATGT CACACTAGCC TACCCAGTAT ATGCACAGCA GAGGCGATGC CCAACTTTCT 1441 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1501 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1561 GTGGAAAGGA CGATTCACCG GAGCCTGAGA TAGGCTAACG CGTTAAGTCg acaatcaacc 1621 tctggattac aaaatttgtg aaagatt