Transcript: Human NM_182687.3

Homo sapiens protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
PKMYT1 (9088)
Length:
2099
CDS:
417..1859

Additional Resources:

NCBI RefSeq record:
NM_182687.3
NBCI Gene record:
PKMYT1 (9088)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146095 GGGCCATGGCTCCTACGGAG pXPR_003 AGG 364 25% 3 0.8618 PKMYT1 PKMYT1 77278
2 BRDN0001146750 AGCAGCGGATGTGTTCAGGT pXPR_003 GGG 871 60% 4 0.4898 PKMYT1 PKMYT1 77280
3 BRDN0001148430 GGGAGAATCCAGGTTCTGCG pXPR_003 TGG 104 7% 3 0.4086 PKMYT1 PKMYT1 77279
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_182687.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000364118 TATGCGGTAAAGCGTTCCATG pLKO_005 822 CDS 100% 4.050 5.670 N PKMYT1 n/a
2 TRCN0000011002 CTATGCGGTAAAGCGTTCCAT pLKO.1 821 CDS 100% 3.000 4.200 N PKMYT1 n/a
3 TRCN0000364117 AGTTCACTGCCGGTCTGTCTT pLKO_005 1384 CDS 100% 4.950 3.465 N PKMYT1 n/a
4 TRCN0000364119 CTCCTACGGAGAGGTCTTCAA pLKO_005 773 CDS 100% 4.950 3.465 N PKMYT1 n/a
5 TRCN0000006237 GCTGCGTTCTGTCCTTGTCAT pLKO.1 1409 CDS 100% 4.950 3.465 N PKMYT1 n/a
6 TRCN0000364110 TGCGTTCTGTCCTTGTCATGA pLKO_005 1411 CDS 100% 4.950 3.465 N PKMYT1 n/a
7 TRCN0000364120 TGTCAAGCCTGCCAACATCTT pLKO_005 1115 CDS 100% 4.950 3.465 N PKMYT1 n/a
8 TRCN0000364112 GAACCTGGATTCTCCCTCAAG pLKO_005 522 CDS 100% 4.050 2.835 N PKMYT1 n/a
9 TRCN0000364109 TGGAAGTGGCATGCAACATGG pLKO_005 1306 CDS 100% 4.050 2.835 N PKMYT1 n/a
10 TRCN0000006236 CCTTGATGTCAAGCCTGCCAA pLKO.1 1109 CDS 100% 2.640 1.848 N PKMYT1 n/a
11 TRCN0000011003 GCCACGCAGAACCTGGATTCT pLKO.1 514 CDS 100% 1.650 1.155 N PKMYT1 n/a
12 TRCN0000006235 CCAGACTCTGCCTCTGCACTT pLKO.1 1958 3UTR 100% 1.350 0.945 N PKMYT1 n/a
13 TRCN0000199578 GCAGCGGATGTGTTCAGTCTG pLKO.1 1272 CDS 100% 1.350 0.945 N PKMYT1 n/a
14 TRCN0000199754 GTCTTCAAGGTGCGCTCCAAG pLKO.1 786 CDS 100% 1.350 0.945 N PKMYT1 n/a
15 TRCN0000199190 CTGAGTTCACTGCCGGTCTGT pLKO.1 1381 CDS 100% 0.880 0.616 N PKMYT1 n/a
16 TRCN0000194658 CTCTGCACTTTTAACCTTTTA pLKO.1 1969 3UTR 100% 0.000 0.000 N PKMYT1 n/a
17 TRCN0000195188 CCTCTGCACTTTTAACCTTTT pLKO.1 1968 3UTR 100% 0.000 0.000 N PKMYT1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_182687.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14928 pDONR223 0% 91.3% 89.5% None 1310_1347del;1440_1441ins95 n/a
2 ccsbBroad304_14928 pLX_304 0% 91.3% 89.5% V5 1310_1347del;1440_1441ins95 n/a
3 TRCN0000469216 ATGAATAGGTGGAGCCTTTTATGT pLX_317 22.4% 91.3% 89.5% V5 1310_1347del;1440_1441ins95 n/a
4 TRCN0000480261 CTTAATGCTATTAAACCTACCCGT pLX_317 21.6% 91.3% 89.5% V5 1310_1347del;1440_1441ins95 n/a
5 TRCN0000489155 TCCCAAGCGGTCGCAATTATAATG pLX_317 21.8% 91.3% 89.5% V5 (not translated due to prior stop codon) 1310_1347del;1440_1441ins95 n/a
6 TRCN0000491634 TGACAAGGGTGTTAACCTTACAAT pLX_317 21.7% 91.2% 89.4% V5 1310_1347del;1440_1441ins96 n/a
7 TRCN0000488460 TATGGCCTAATCTGGAGGTTGCCT pLX_317 21.9% 90.9% 88.9% V5 (not translated due to prior stop codon) 4_9delCTAGAA;1310_1347del;1440_1441ins95 n/a
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