Transcript: Human NM_198216.2

Homo sapiens small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-07-10
Taxon:
Homo sapiens (human)
Gene:
SNRPB (6628)
Length:
936
CDS:
100..822

Additional Resources:

NCBI RefSeq record:
NM_198216.2
NBCI Gene record:
SNRPB (6628)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_198216.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000072476 CCTCCCAAAGATACTGGTATT pLKO.1 355 CDS 100% 10.800 7.560 N SNRPB n/a
2 TRCN0000286839 CCTCCCAAAGATACTGGTATT pLKO_005 355 CDS 100% 10.800 7.560 N SNRPB n/a
3 TRCN0000294241 AGCCAAAGAACTCCAAACAAG pLKO_005 254 CDS 100% 4.950 3.465 N SNRPB n/a
4 TRCN0000072475 CCACAAGGAAGAGGTACTGTT pLKO.1 529 CDS 100% 4.950 3.465 N SNRPB n/a
5 TRCN0000286762 CCACAAGGAAGAGGTACTGTT pLKO_005 529 CDS 100% 4.950 3.465 N SNRPB n/a
6 TRCN0000072474 TCCTGGTATGAGACCTCCTAT pLKO.1 675 CDS 100% 4.950 3.465 N SNRPB n/a
7 TRCN0000294223 TCTGGTCTCAATGACAGTAGA pLKO_005 327 CDS 100% 4.950 3.465 N SNRPB n/a
8 TRCN0000109269 TCCAAACAAGCAGAAAGGGAA pLKO.1 265 CDS 100% 2.640 1.848 N Snrpb n/a
9 TRCN0000309857 TCCAAACAAGCAGAAAGGGAA pLKO_005 265 CDS 100% 2.640 1.848 N Snrpb n/a
10 TRCN0000072477 CACATGAATTTGATCCTCTGT pLKO.1 208 CDS 100% 2.640 1.584 N SNRPB n/a
11 TRCN0000327720 CACATGAATTTGATCCTCTGT pLKO_005 208 CDS 100% 2.640 1.584 N SNRPB n/a
12 TRCN0000072473 GCAGCATATTGATTACAGGAT pLKO.1 129 CDS 100% 2.640 1.584 N SNRPB n/a
13 TRCN0000075137 CAGAAAGATCAAGCCAAAGAA pLKO.1 243 CDS 100% 5.625 2.813 Y SNRPN n/a
14 TRCN0000075136 GTTCAGAAAGATCAAGCCAAA pLKO.1 240 CDS 100% 4.050 2.025 Y SNRPN n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_198216.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01568 pDONR223 100% 95.6% 95.4% None (many diffs) n/a
2 ccsbBroad304_01568 pLX_304 0% 95.6% 95.4% V5 (many diffs) n/a
3 TRCN0000473898 TGTCAGCATTCTACCTATAACCAG pLX_317 51% 95.6% 95.4% V5 (many diffs) n/a
4 ccsbBroadEn_01575 pDONR223 100% 82% 92.9% None (many diffs) n/a
5 ccsbBroad304_01575 pLX_304 0% 82% 92.9% V5 (many diffs) n/a
6 TRCN0000471606 ATAAAGGACCTTCCAAGGTTCAAG pLX_317 64.7% 81.9% 33.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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