Construct: ORF TRCN0000471893
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016680.1_s317c1
- Derived from:
- ccsbBroadEn_11498
- DNA Barcode:
- GGGCTAAATTTTAGCTTGGTCAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TIMM23B (653252)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471893
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100287932 | TIMM23 | translocase of inner mitoch... | NM_006327.4 | 99.5% | 99.5% | 182_184delTTT |
| 2 | human | 100652748 | TIMM23B | translocase of inner mitoch... | NM_001290117.1 | 85.8% | 80.3% | (many diffs) |
| 3 | human | 100652748 | TIMM23B | translocase of inner mitoch... | NM_001290118.1 | 63.1% | 57.8% | (many diffs) |
| 4 | human | 100652748 | TIMM23B | translocase of inner mitoch... | NM_001365871.1 | 47.4% | 43.2% | (many diffs) |
| 5 | human | 100287932 | TIMM23 | translocase of inner mitoch... | NR_073029.1 | 45.7% | (many diffs) | |
| 6 | human | 100287932 | TIMM23 | translocase of inner mitoch... | NR_073030.1 | 45.6% | (many diffs) | |
| 7 | human | 100652748 | TIMM23B | translocase of inner mitoch... | NR_110767.1 | 21.6% | (many diffs) | |
| 8 | human | 113218477 | TIMM23B-AGAP6 | TIMM23B-AGAP6 readthrough | NR_158656.1 | 19.5% | (many diffs) | |
| 9 | human | 100652748 | TIMM23B | translocase of inner mitoch... | NR_158651.1 | 19.1% | (many diffs) | |
| 10 | human | 113218477 | TIMM23B-AGAP6 | TIMM23B-AGAP6 readthrough | NR_158657.1 | 18.7% | (many diffs) | |
| 11 | human | 113218477 | TIMM23B-AGAP6 | TIMM23B-AGAP6 readthrough | NR_158654.1 | 17.5% | (many diffs) | |
| 12 | human | 113218477 | TIMM23B-AGAP6 | TIMM23B-AGAP6 readthrough | NR_158660.1 | 17.3% | (many diffs) | |
| 13 | human | 100652748 | TIMM23B | translocase of inner mitoch... | NR_158652.1 | 17.1% | (many diffs) | |
| 14 | human | 113218477 | TIMM23B-AGAP6 | TIMM23B-AGAP6 readthrough | NR_158659.1 | 16.5% | (many diffs) | |
| 15 | human | 113218477 | TIMM23B-AGAP6 | TIMM23B-AGAP6 readthrough | NR_158658.1 | 16.2% | (many diffs) | |
| 16 | human | 113218477 | TIMM23B-AGAP6 | TIMM23B-AGAP6 readthrough | NR_158661.1 | 15.1% | (many diffs) | |
| 17 | mouse | 53600 | Timm23 | translocase of inner mitoch... | NM_016897.3 | 90.1% | 96.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 690
- ORF length:
- 624
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aggaggcggg ggaagcggca acaaaaccac agggggattg gccggctttt 121 tcggagccgg cggagcaggt tactcgcacg cggatttggc tggcgtcccg ctaactggta 181 tgaaccctct gtctccttat ttaaatgtgg atccacgata ccTCGTGCAG GATACAGATG 241 AGTTTATACC TACCGGAGCT AATAAAACCC GGGGCAGATT TGAGCTGGCC TTCTTTACGA 301 TTGGAGGATG TTGCATGACA GGGGCTGCGT TTGGTGCAAT GAATGGTCTT CGGCTAGGAT 361 TGAAGGAAAC CCAGAACATG GCCTGGTCCA AACCAAGAAA TGTACAGATT TTGAATATGG 421 TGACTAGGCA AGGGGCACTT TGGGCTAATA CTCTAGGTTC TCTGGCTTTG CTCTATAGTG 481 CATTTGGTGT CATCATTGAG AAAACACGAG GTGCAGAAGA TGACCTTAAC ACAGTAGCAG 541 CTGGAACCAT GACAGGCATG TTGTATAAAT GTACAGGTGG TCTTCGAGGG ATAGCACGAG 601 GTGGTCTGAC AGGACTAACA CTTACCAGCC TCTATGCACT ATATAATAAC TGGGAGCACA 661 TGAAAGGCTC CTTGCTCCAA CAGTCACTCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 721 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 781 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGGCT 841 AAATTTTAGC TTGGTCAGCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 901 tgaaagatt