Transcript: Human NR_146196.2

Homo sapiens GRAM domain containing 2B (GRAMD2B), transcript variant 11, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
GRAMD2B (65983)
Length:
3055
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_146196.2
NBCI Gene record:
GRAMD2B (65983)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_146196.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000137123 CCATCGGAATACCTCATGTTT pLKO.1 1666 3UTR 100% 5.625 7.875 N GRAMD2B n/a
2 TRCN0000137920 GCCATCGGAATACCTCATGTT pLKO.1 1665 3UTR 100% 4.950 6.930 N GRAMD2B n/a
3 TRCN0000138479 CGAGATTTCCATGCGACAGAA pLKO.1 1042 3UTR 100% 4.950 6.435 N GRAMD2B n/a
4 TRCN0000137081 CGAACACACTCTCTTGGATAA pLKO.1 1922 3UTR 100% 10.800 7.560 N GRAMD2B n/a
5 TRCN0000133843 CCTGTTATGTTCTGGTTGAAA pLKO.1 2185 3UTR 100% 5.625 3.938 N GRAMD2B n/a
6 TRCN0000134066 GTCAGAAACTGTTGGAATCTT pLKO.1 1173 3UTR 100% 5.625 3.938 N GRAMD2B n/a
7 TRCN0000137676 GCGAACACACTCTCTTGGATA pLKO.1 1921 3UTR 100% 4.950 3.465 N GRAMD2B n/a
8 TRCN0000136589 CCCAAACAGTTCTGAATGTCT pLKO.1 1064 3UTR 100% 3.000 2.100 N GRAMD2B n/a
9 TRCN0000137483 GCAGATGAGAATCCAGACATT pLKO.1 1751 3UTR 100% 4.950 2.970 N GRAMD2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_146196.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04000 pDONR223 100% 42.4% None 1_198del;1454_1603del;1645_3055del n/a
2 ccsbBroad304_04000 pLX_304 0% 42.4% V5 1_198del;1454_1603del;1645_3055del n/a
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