Construct: ORF ccsbBroadEn_04000

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009705.1_s300c1, BRDN0000385260
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRAMD2B (65983)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 65983 GRAMD2B GRAM domain containing 2B NM_023927.4 100% 100%
2 human 65983 GRAMD2B GRAM domain containing 2B NM_001349541.2 99.7% 99.7% 846_848delAGT
3 human 65983 GRAMD2B GRAM domain containing 2B NM_001146321.2 95.3% 84.3% 0_1insATGACTGAACTACAGCAAG;185_227del
4 human 65983 GRAMD2B GRAM domain containing 2B NM_001349544.2 95.1% 84.1% 0_1insATGACTGAACTACAGCAAG;185_227del;870_872delAGT
5 human 65983 GRAMD2B GRAM domain containing 2B NM_001146322.3 94.7% 94.6% (many diffs)
6 human 65983 GRAMD2B GRAM domain containing 2B XM_005272063.3 94.7% 94.6% (many diffs)
7 human 65983 GRAMD2B GRAM domain containing 2B NM_001146319.3 94.6% 92.1% (many diffs)
8 human 65983 GRAMD2B GRAM domain containing 2B NM_001349542.2 94.5% 94.4% (many diffs)
9 human 65983 GRAMD2B GRAM domain containing 2B XM_005272062.4 94.5% 94.4% (many diffs)
10 human 65983 GRAMD2B GRAM domain containing 2B XM_011543594.2 94.5% 94.4% (many diffs)
11 human 65983 GRAMD2B GRAM domain containing 2B XM_011543593.2 94.4% 91.9% (many diffs)
12 human 65983 GRAMD2B GRAM domain containing 2B XM_005272058.4 92.7% 92.1% (many diffs)
13 human 65983 GRAMD2B GRAM domain containing 2B XM_005272057.4 92.5% 91.9% (many diffs)
14 human 65983 GRAMD2B GRAM domain containing 2B XM_017009782.1 89.5% 82.7% 0_1ins97;107_149del
15 human 65983 GRAMD2B GRAM domain containing 2B XM_005272064.3 89.3% 82.5% 0_1ins97;107_149del;792_794delAGT
16 human 65983 GRAMD2B GRAM domain containing 2B XM_017009781.1 89.3% 82.5% 0_1ins97;107_149del;792_794delAGT
17 human 65983 GRAMD2B GRAM domain containing 2B XM_024446201.1 89.3% 82.5% 0_1ins97;107_149del;792_794delAGT
18 human 65983 GRAMD2B GRAM domain containing 2B XM_011543592.3 84.7% 75.2% (many diffs)
19 human 65983 GRAMD2B GRAM domain containing 2B XM_011543591.2 84.6% 75% (many diffs)
20 human 65983 GRAMD2B GRAM domain containing 2B NM_001146320.2 75.9% 75.6% 0_1ins302;2_3insCTTCCAGCCA
21 human 65983 GRAMD2B GRAM domain containing 2B XM_017009785.1 75.9% 75.6% 0_1ins302;2_3insCTTCCAGCCA
22 human 65983 GRAMD2B GRAM domain containing 2B NM_001349543.2 75.7% 75.5% 0_1ins302;2_3insCTTCCAGCCA;534_536delAGT
23 human 65983 GRAMD2B GRAM domain containing 2B XM_017009784.1 75.7% 75.5% 0_1ins302;2_3insCTTCCAGCCA;534_536delAGT
24 human 65983 GRAMD2B GRAM domain containing 2B XM_017009783.1 72.8% 71.5% (many diffs)
25 human 65983 GRAMD2B GRAM domain containing 2B NR_146196.2 42.4% 1_198del;1454_1603del;1645_3055del
26 human 65983 GRAMD2B GRAM domain containing 2B NR_146195.2 41.8% (many diffs)
27 human 65983 GRAMD2B GRAM domain containing 2B NR_146197.2 38.7% (many diffs)
28 mouse 107022 Gramd3 GRAM domain containing 3 NM_026240.2 85.7% 86.5% (many diffs)
29 mouse 107022 Gramd3 GRAM domain containing 3 XM_006525494.1 81.8% 78.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1362
ORF length:
1296
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGAATGAC TGAACTACAG CAAGATGTGG AAGACACAAA GCCTGCGAAA GTGCTCGGGA
121AGAGGGAGAG CAAACTTGGC TCAGCCCACT CAGAGGCTGA GAATGGTGTG GAGGAGAAAA
181AGAAAGCCTG CAGGTCGCCA ACAGCCCAAT CCCCTACCCC ATCTGTGGAG GCGGACTCCC
241CAGACCAGAA GAAAATCATT AGCCTATGGT CAAAATCCAG TTTTGATGGT GCCTCTTTAG
301CAAGTGATAA GAACGACTGT AAAACAGAAA GCAAAAATGA CCCTAAGACT GAAAGAAAAA
361AGTCTTCATC TTCCAGCCAG TACAAGGCCA ATATGCACTT TCACAAGTTG TTTCTTAGTG
421TCCCAACGGA GGAACCACTG AAGCAAAGCT TTACCTGTGC TCTACAGAAA GAAATACTAT
481ACCAAGGAAA GCTCTTTGTA TCAGAAAACT GGATTTGTTT TCATTCCAAA GTCTTTGGAA
541AAGACACAAA GATCTCTATT CCAGCTTTCT CGGTAACCCT AATAAAGAAA ACCAAAACTG
601CTCTTCTAGT GCCAAACGCC CTGATCATAG CAACAGTCAC AGACAGGTAC ATATTTGTCT
661CCTTACTCTC CAGAGATTCA ACTTACAAAC TACTAAAATC TGTGTGTGGA CACTTAGAAA
721ATACAAGTGT TGGTAACAGT CCCAATCCAT CTTCTGCTGA AAACAGTTTC CGAGCAGACC
781GCCCTTCATC TCTGCCTCTG GATTTCAATG ATGAATTCTC AGATCTGGAT GGAGTGGTTC
841AACAAAGAAG GCAAGACATG GAAGGATATA GCAGTTCTGG TTCTCAAACT CCTGAATCTG
901AGAACTCTCG AGATTTCCAT GCGACAGAAT CCCAAACAGT TCTGAATGTC TCCAAGGGAG
961AAGCAAAGCC AACTCGGGCA GATGCCCATG TGAACAGAGT ACCTGAAGGA AAAGCCAAGA
1021GTCTCCCTGT ACAGGGTCTG TCAGAAACTG TTGGAATCTT ACATAAAGTC AAGTCTCAGA
1081AATGTCCGAT GCTTCACCAT ATTCTTATAT TCTATGCAAT TGTTGTCTGT GCACTAATCA
1141TCTCGACCTT CTACATGAGA TACAGAATTA ATACTCTGGA GGAGCAGCTG GGGTTACTAA
1201CCTCCATTGT GGACACCCAT AATACTGAAC AGGCAGCACC ATCTGGCCTG AGGTCACAAG
1261TACAATTCAA TGTGGAGGTT CTCTGTCAAG AGCTTACAGC TAACATAGTG AAATTAGAAA
1321AGATACAAAA TAACTTACAG AAGTTGCTTG AGAATGGTGA CTGCCCAACT TTCTTGTACA
1381AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac

Download FASTA (ORF) (Full)