Construct: ORF ccsbBroadEn_04000
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009705.1_s300c1, BRDN0000385260
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRAMD2B (65983)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 65983 | GRAMD2B | GRAM domain containing 2B | NM_023927.4 | 100% | 100% | |
2 | human | 65983 | GRAMD2B | GRAM domain containing 2B | NM_001349541.2 | 99.7% | 99.7% | 846_848delAGT |
3 | human | 65983 | GRAMD2B | GRAM domain containing 2B | NM_001146321.2 | 95.3% | 84.3% | 0_1insATGACTGAACTACAGCAAG;185_227del |
4 | human | 65983 | GRAMD2B | GRAM domain containing 2B | NM_001349544.2 | 95.1% | 84.1% | 0_1insATGACTGAACTACAGCAAG;185_227del;870_872delAGT |
5 | human | 65983 | GRAMD2B | GRAM domain containing 2B | NM_001146322.3 | 94.7% | 94.6% | (many diffs) |
6 | human | 65983 | GRAMD2B | GRAM domain containing 2B | XM_005272063.3 | 94.7% | 94.6% | (many diffs) |
7 | human | 65983 | GRAMD2B | GRAM domain containing 2B | NM_001146319.3 | 94.6% | 92.1% | (many diffs) |
8 | human | 65983 | GRAMD2B | GRAM domain containing 2B | NM_001349542.2 | 94.5% | 94.4% | (many diffs) |
9 | human | 65983 | GRAMD2B | GRAM domain containing 2B | XM_005272062.4 | 94.5% | 94.4% | (many diffs) |
10 | human | 65983 | GRAMD2B | GRAM domain containing 2B | XM_011543594.2 | 94.5% | 94.4% | (many diffs) |
11 | human | 65983 | GRAMD2B | GRAM domain containing 2B | XM_011543593.2 | 94.4% | 91.9% | (many diffs) |
12 | human | 65983 | GRAMD2B | GRAM domain containing 2B | XM_005272058.4 | 92.7% | 92.1% | (many diffs) |
13 | human | 65983 | GRAMD2B | GRAM domain containing 2B | XM_005272057.4 | 92.5% | 91.9% | (many diffs) |
14 | human | 65983 | GRAMD2B | GRAM domain containing 2B | XM_017009782.1 | 89.5% | 82.7% | 0_1ins97;107_149del |
15 | human | 65983 | GRAMD2B | GRAM domain containing 2B | XM_005272064.3 | 89.3% | 82.5% | 0_1ins97;107_149del;792_794delAGT |
16 | human | 65983 | GRAMD2B | GRAM domain containing 2B | XM_017009781.1 | 89.3% | 82.5% | 0_1ins97;107_149del;792_794delAGT |
17 | human | 65983 | GRAMD2B | GRAM domain containing 2B | XM_024446201.1 | 89.3% | 82.5% | 0_1ins97;107_149del;792_794delAGT |
18 | human | 65983 | GRAMD2B | GRAM domain containing 2B | XM_011543592.3 | 84.7% | 75.2% | (many diffs) |
19 | human | 65983 | GRAMD2B | GRAM domain containing 2B | XM_011543591.2 | 84.6% | 75% | (many diffs) |
20 | human | 65983 | GRAMD2B | GRAM domain containing 2B | NM_001146320.2 | 75.9% | 75.6% | 0_1ins302;2_3insCTTCCAGCCA |
21 | human | 65983 | GRAMD2B | GRAM domain containing 2B | XM_017009785.1 | 75.9% | 75.6% | 0_1ins302;2_3insCTTCCAGCCA |
22 | human | 65983 | GRAMD2B | GRAM domain containing 2B | NM_001349543.2 | 75.7% | 75.5% | 0_1ins302;2_3insCTTCCAGCCA;534_536delAGT |
23 | human | 65983 | GRAMD2B | GRAM domain containing 2B | XM_017009784.1 | 75.7% | 75.5% | 0_1ins302;2_3insCTTCCAGCCA;534_536delAGT |
24 | human | 65983 | GRAMD2B | GRAM domain containing 2B | XM_017009783.1 | 72.8% | 71.5% | (many diffs) |
25 | human | 65983 | GRAMD2B | GRAM domain containing 2B | NR_146196.2 | 42.4% | 1_198del;1454_1603del;1645_3055del | |
26 | human | 65983 | GRAMD2B | GRAM domain containing 2B | NR_146195.2 | 41.8% | (many diffs) | |
27 | human | 65983 | GRAMD2B | GRAM domain containing 2B | NR_146197.2 | 38.7% | (many diffs) | |
28 | mouse | 107022 | Gramd3 | GRAM domain containing 3 | NM_026240.2 | 85.7% | 86.5% | (many diffs) |
29 | mouse | 107022 | Gramd3 | GRAM domain containing 3 | XM_006525494.1 | 81.8% | 78.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1362
- ORF length:
- 1296
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGAATGAC TGAACTACAG CAAGATGTGG AAGACACAAA GCCTGCGAAA GTGCTCGGGA 121 AGAGGGAGAG CAAACTTGGC TCAGCCCACT CAGAGGCTGA GAATGGTGTG GAGGAGAAAA 181 AGAAAGCCTG CAGGTCGCCA ACAGCCCAAT CCCCTACCCC ATCTGTGGAG GCGGACTCCC 241 CAGACCAGAA GAAAATCATT AGCCTATGGT CAAAATCCAG TTTTGATGGT GCCTCTTTAG 301 CAAGTGATAA GAACGACTGT AAAACAGAAA GCAAAAATGA CCCTAAGACT GAAAGAAAAA 361 AGTCTTCATC TTCCAGCCAG TACAAGGCCA ATATGCACTT TCACAAGTTG TTTCTTAGTG 421 TCCCAACGGA GGAACCACTG AAGCAAAGCT TTACCTGTGC TCTACAGAAA GAAATACTAT 481 ACCAAGGAAA GCTCTTTGTA TCAGAAAACT GGATTTGTTT TCATTCCAAA GTCTTTGGAA 541 AAGACACAAA GATCTCTATT CCAGCTTTCT CGGTAACCCT AATAAAGAAA ACCAAAACTG 601 CTCTTCTAGT GCCAAACGCC CTGATCATAG CAACAGTCAC AGACAGGTAC ATATTTGTCT 661 CCTTACTCTC CAGAGATTCA ACTTACAAAC TACTAAAATC TGTGTGTGGA CACTTAGAAA 721 ATACAAGTGT TGGTAACAGT CCCAATCCAT CTTCTGCTGA AAACAGTTTC CGAGCAGACC 781 GCCCTTCATC TCTGCCTCTG GATTTCAATG ATGAATTCTC AGATCTGGAT GGAGTGGTTC 841 AACAAAGAAG GCAAGACATG GAAGGATATA GCAGTTCTGG TTCTCAAACT CCTGAATCTG 901 AGAACTCTCG AGATTTCCAT GCGACAGAAT CCCAAACAGT TCTGAATGTC TCCAAGGGAG 961 AAGCAAAGCC AACTCGGGCA GATGCCCATG TGAACAGAGT ACCTGAAGGA AAAGCCAAGA 1021 GTCTCCCTGT ACAGGGTCTG TCAGAAACTG TTGGAATCTT ACATAAAGTC AAGTCTCAGA 1081 AATGTCCGAT GCTTCACCAT ATTCTTATAT TCTATGCAAT TGTTGTCTGT GCACTAATCA 1141 TCTCGACCTT CTACATGAGA TACAGAATTA ATACTCTGGA GGAGCAGCTG GGGTTACTAA 1201 CCTCCATTGT GGACACCCAT AATACTGAAC AGGCAGCACC ATCTGGCCTG AGGTCACAAG 1261 TACAATTCAA TGTGGAGGTT CTCTGTCAAG AGCTTACAGC TAACATAGTG AAATTAGAAA 1321 AGATACAAAA TAACTTACAG AAGTTGCTTG AGAATGGTGA CTGCCCAACT TTCTTGTACA 1381 AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac