Transcript: Mouse NM_001177759.1

Mus musculus tumor necrosis factor, alpha-induced protein 8 (Tnfaip8), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Tnfaip8 (106869)
Length:
3230
CDS:
200..766

Additional Resources:

NCBI RefSeq record:
NM_001177759.1
NBCI Gene record:
Tnfaip8 (106869)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001177759.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000348693 AGTCTCACGGACGGGTTAATA pLKO_005 618 CDS 100% 15.000 21.000 N Tnfaip8 n/a
2 TRCN0000100261 CGGACGGGTTAATAATGTCTT pLKO.1 625 CDS 100% 4.950 6.930 N Tnfaip8 n/a
3 TRCN0000100262 GCTGCCTTGTACAATCCCTTT pLKO.1 674 CDS 100% 4.050 3.240 N Tnfaip8 n/a
4 TRCN0000351747 GCTGCCTTGTACAATCCCTTT pLKO_005 674 CDS 100% 4.050 3.240 N Tnfaip8 n/a
5 TRCN0000375643 TGGCTACAGATGTCTTCAATT pLKO_005 201 CDS 100% 13.200 9.240 N Tnfaip8 n/a
6 TRCN0000375642 AGCTCCTACACGAGATCATTC pLKO_005 579 CDS 100% 10.800 7.560 N Tnfaip8 n/a
7 TRCN0000100264 CCACAGGAACAATCAGTTCAA pLKO.1 424 CDS 100% 4.950 3.465 N Tnfaip8 n/a
8 TRCN0000100263 CCTCCACAGGAACAATCAGTT pLKO.1 421 CDS 100% 4.950 3.465 N Tnfaip8 n/a
9 TRCN0000367650 CCTCCACAGGAACAATCAGTT pLKO_005 421 CDS 100% 4.950 3.465 N Tnfaip8 n/a
10 TRCN0000100260 CCTGTTATTGTTGTTGGACAT pLKO.1 892 3UTR 100% 4.050 2.835 N Tnfaip8 n/a
11 TRCN0000351748 CCTGTTATTGTTGTTGGACAT pLKO_005 892 3UTR 100% 4.050 2.835 N Tnfaip8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001177759.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000472974 GCGAACAGCTTTCGCACATATTAC pLX_317 88.5% 81.6% 89.3% V5 (many diffs) n/a
2 ccsbBroadEn_14094 pDONR223 100% 81.4% 86.8% None (many diffs) n/a
3 ccsbBroad304_14094 pLX_304 0% 81.4% 86.8% V5 (not translated due to frame shift) (many diffs) n/a
4 ccsbBroadEn_07947 pDONR223 100% 81.4% 88.8% None (many diffs) n/a
5 ccsbBroad304_07947 pLX_304 0% 81.4% 88.8% V5 (many diffs) n/a
6 TRCN0000468140 CCTAGATTGTGCATGTAGGAACAA pLX_317 49% 81.4% 88.8% V5 (many diffs) n/a
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