Transcript: Human XM_011529410.1

PREDICTED: Homo sapiens Ras association domain family member 2 (RASSF2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RASSF2 (9770)
Length:
5427
CDS:
191..1177

Additional Resources:

NCBI RefSeq record:
XM_011529410.1
NBCI Gene record:
RASSF2 (9770)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011529410.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000077888 GCGTGCAATGAACCTGAAATT pLKO.1 3646 3UTR 100% 13.200 18.480 N RASSF2 n/a
2 TRCN0000077645 CCAGTATATAAAGTTCGAGAT pLKO.1 1015 CDS 100% 4.050 5.670 N Rassf2 n/a
3 TRCN0000077892 GCCACCGATTACCCGCTGATT pLKO.1 905 CDS 100% 1.650 2.310 N RASSF2 n/a
4 TRCN0000077891 TCTGAAGACCTACAACTTGTA pLKO.1 268 CDS 100% 4.950 3.960 N RASSF2 n/a
5 TRCN0000077889 GAAGACCTACAACTTGTACTA pLKO.1 271 CDS 100% 4.950 3.465 N RASSF2 n/a
6 TRCN0000116737 CCTCCCAAGTAGCTGGAATTA pLKO.1 4583 3UTR 100% 13.200 6.600 Y CLDN18 n/a
7 TRCN0000008902 CCTCCCAAAGTGTTGGGATTA pLKO.1 4720 3UTR 100% 1.080 0.540 Y GPR83 n/a
8 TRCN0000156315 CCTCCCAAAGTGTTGGGATTA pLKO.1 4720 3UTR 100% 1.080 0.540 Y MYORG n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011529410.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02239 pDONR223 100% 99.3% 99% None 377_382delGTACAT n/a
2 ccsbBroad304_02239 pLX_304 0% 99.3% 99% V5 377_382delGTACAT n/a
3 TRCN0000472024 CCTCTAGCTTAATACGCTCCCAGT pLX_317 43% 99.3% 99% V5 377_382delGTACAT n/a
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