Transcript: Mouse XM_006521163.2

PREDICTED: Mus musculus NEL-like 2 (Nell2), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Nell2 (54003)
Length:
3287
CDS:
174..2633

Additional Resources:

NCBI RefSeq record:
XM_006521163.2
NBCI Gene record:
Nell2 (54003)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006521163.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000054311 CCCTACAGATTGACGTCTTAA pLKO.1 262 CDS 100% 13.200 18.480 N NELL2 n/a
2 TRCN0000216636 CCCTACAGATTGACGTCTTAA pLKO.1 262 CDS 100% 13.200 18.480 N Nell2 n/a
3 TRCN0000250093 CCCTACAGATTGACGTCTTAA pLKO_005 262 CDS 100% 13.200 18.480 N Nell2 n/a
4 TRCN0000217156 CCACTTAAATTCGGGAGTTAT pLKO.1 470 CDS 100% 13.200 10.560 N Nell2 n/a
5 TRCN0000250091 CCACTTAAATTCGGGAGTTAT pLKO_005 470 CDS 100% 13.200 10.560 N Nell2 n/a
6 TRCN0000250094 ACAGCGGTGAGCGAGTTATAT pLKO_005 3034 3UTR 100% 15.000 10.500 N Nell2 n/a
7 TRCN0000250092 ACGGAAGTGTTTCCTTATATT pLKO_005 594 CDS 100% 15.000 10.500 N Nell2 n/a
8 TRCN0000184522 GCGGAGAATCCTGTGAAGATA pLKO.1 1969 CDS 100% 5.625 3.938 N Nell2 n/a
9 TRCN0000183899 CCAACATGCAACGACTTCCAT pLKO.1 867 CDS 100% 3.000 2.100 N Nell2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006521163.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01083 pDONR223 100% 86.1% 93.6% None (many diffs) n/a
2 ccsbBroad304_01083 pLX_304 0% 86.1% 93.6% V5 (many diffs) n/a
3 TRCN0000492312 GCTGTTCGGTAGTCACGTTCATAA pLX_317 6.5% 86.1% 93.6% V5 (many diffs) n/a
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