Transcript: Mouse XM_011242702.1

PREDICTED: Mus musculus poly (ADP-ribose) polymerase family, member 16 (Parp16), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Parp16 (214424)
Length:
2323
CDS:
331..1092

Additional Resources:

NCBI RefSeq record:
XM_011242702.1
NBCI Gene record:
Parp16 (214424)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011242702.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000336408 GTACTTTGTAGTCACCAATAA pLKO_005 882 CDS 100% 13.200 18.480 N Parp16 n/a
2 TRCN0000336342 CAAGAGGGACTGTCGTGTTTC pLKO_005 1543 3UTR 100% 10.800 8.640 N Parp16 n/a
3 TRCN0000190801 GCCTCTTTCTGGTGTTGTTAT pLKO.1 1911 3UTR 100% 13.200 9.240 N Parp16 n/a
4 TRCN0000336407 TGAAGTACCTGCTGGTGTATT pLKO_005 917 CDS 100% 13.200 9.240 N Parp16 n/a
5 TRCN0000200923 CAAGTGCCAAATCAAGAAGAA pLKO.1 792 CDS 100% 4.950 3.465 N Parp16 n/a
6 TRCN0000190330 CGGAACCAAATGTTTGCGTTT pLKO.1 1795 3UTR 100% 4.050 2.835 N Parp16 n/a
7 TRCN0000336341 ACTTGAGCCTGGCCCTCATTT pLKO_005 680 CDS 100% 13.200 7.920 N Parp16 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011242702.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12121 pDONR223 100% 68.1% 69.8% None (many diffs) n/a
2 ccsbBroad304_12121 pLX_304 0% 68.1% 69.8% V5 (many diffs) n/a
3 TRCN0000467473 TGCCGCCCGGAACTAGAAACGCGG pLX_317 27.1% 68.1% 69.8% V5 (many diffs) n/a
4 ccsbBroadEn_03496 pDONR223 100% 68.1% 69.9% None (many diffs) n/a
5 ccsbBroad304_03496 pLX_304 0% 68.1% 69.9% V5 (many diffs) n/a
6 TRCN0000471995 GTGACGTGGTTTACTCTACAAATT pLX_317 35.6% 68.1% 69.9% V5 (many diffs) n/a
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