Transcript: Human XR_001745491.1

PREDICTED: Homo sapiens epoxide hydrolase 2 (EPHX2), transcript variant X1, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
EPHX2 (2053)
Length:
2420
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001745491.1
NBCI Gene record:
EPHX2 (2053)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001745491.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000439761 GTCAGGTGGGAATGGTCAAAC pLKO_005 519 3UTR 100% 10.800 15.120 N EPHX2 n/a
2 TRCN0000050554 CCGGCTTATGAAAGGAGAGAT pLKO.1 211 3UTR 100% 4.950 6.930 N EPHX2 n/a
3 TRCN0000414811 CCATGGGTACGTGACAGTAAA pLKO_005 772 3UTR 100% 13.200 10.560 N EPHX2 n/a
4 TRCN0000417482 ATATTGCATGGAAGTGTTATG pLKO_005 979 3UTR 100% 10.800 7.560 N EPHX2 n/a
5 TRCN0000443272 CAGAACCTGAGTCGGACTTTC pLKO_005 1253 3UTR 100% 10.800 7.560 N EPHX2 n/a
6 TRCN0000437939 GTCATCTGCTCCTCCCGAAAT pLKO_005 952 3UTR 100% 10.800 7.560 N EPHX2 n/a
7 TRCN0000050556 CTGGTTTCAGAGGTCCTCTAA pLKO.1 1428 3UTR 100% 4.950 3.465 N EPHX2 n/a
8 TRCN0000050555 GAAATCTTTGACAAGGCGATT pLKO.1 326 3UTR 100% 4.050 2.835 N EPHX2 n/a
9 TRCN0000050553 GCTATGGACATGAAAGGCTAT pLKO.1 926 3UTR 100% 4.050 2.835 N EPHX2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001745491.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00510 pDONR223 100% 67.7% None (many diffs) n/a
2 ccsbBroad304_00510 pLX_304 0% 67.7% V5 (many diffs) n/a
3 TRCN0000476514 AATTCTGTCTGTGTGGCATGGTGT pLX_317 21.6% 67.7% V5 (many diffs) n/a
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