Transcript: Mouse XM_011244652.2

PREDICTED: Mus musculus FCH domain only 2 (Fcho2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Fcho2 (218503)
Length:
4634
CDS:
215..2569

Additional Resources:

NCBI RefSeq record:
XM_011244652.2
NBCI Gene record:
Fcho2 (218503)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011244652.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000216673 CTGATGCAGATTCGCTTAATA pLKO.1 1062 CDS 100% 15.000 21.000 N Fcho2 n/a
2 TRCN0000190729 GCGCATCCAAATAACTTGCAT pLKO.1 1223 CDS 100% 3.000 4.200 N Fcho2 n/a
3 TRCN0000216197 CAACTTACTTCTGCGTTAATT pLKO.1 3201 3UTR 100% 15.000 10.500 N Fcho2 n/a
4 TRCN0000216613 GTAGTAACAAGCCTAACATTT pLKO.1 2860 3UTR 100% 13.200 9.240 N Fcho2 n/a
5 TRCN0000191031 CCTGGTCTTATTGAATTTGAA pLKO.1 932 CDS 100% 5.625 3.938 N Fcho2 n/a
6 TRCN0000191832 GCTATAGACTTTCCTTAGTAA pLKO.1 2508 CDS 100% 5.625 3.938 N Fcho2 n/a
7 TRCN0000201357 GCTTCTTTGGATGAACTGAAA pLKO.1 1253 CDS 100% 4.950 3.465 N Fcho2 n/a
8 TRCN0000190935 CCAGGTATCATCAAATGGCAT pLKO.1 2113 CDS 100% 2.640 1.848 N Fcho2 n/a
9 TRCN0000191494 GCACAGAAGTTATTTAGCTTT pLKO.1 2892 3UTR 100% 4.950 2.970 N Fcho2 n/a
10 TRCN0000448675 AGCTGACTACAAACATTAAAT pLKO_005 2685 3UTR 100% 15.000 9.000 N FCHO2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011244652.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14358 pDONR223 100% 36.9% 33.7% None (many diffs) n/a
2 ccsbBroad304_14358 pLX_304 0% 36.9% 33.7% V5 (many diffs) n/a
3 TRCN0000472736 ATTGGGATTGACGTTGCAACCCCG pLX_317 52.5% 36.9% 33.7% V5 (many diffs) n/a
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