Transcript: Mouse XM_006529213.3

PREDICTED: Mus musculus leucine rich repeat (in FLII) interacting protein 1 (Lrrfip1), transcript variant X35, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Lrrfip1 (16978)
Length:
2866
CDS:
310..2118

Additional Resources:

NCBI RefSeq record:
XM_006529213.3
NBCI Gene record:
Lrrfip1 (16978)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006529213.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000340251 ACATGGAATAATCCTAAATTC pLKO_005 1515 CDS 100% 13.200 18.480 N Lrrfip1 n/a
2 TRCN0000340185 ACGGTTATGACGGAGACTATT pLKO_005 698 CDS 100% 13.200 18.480 N Lrrfip1 n/a
3 TRCN0000340252 AGGCGATGGTCTAGACGTAAA pLKO_005 2612 3UTR 100% 10.800 15.120 N Lrrfip1 n/a
4 TRCN0000340187 GAGCGCTACTCTCGTAGATTC pLKO_005 568 CDS 100% 10.800 15.120 N Lrrfip1 n/a
5 TRCN0000351054 CAGATCAGCGACCTCAAATTT pLKO_005 1837 CDS 100% 15.000 12.000 N Lrrfip1 n/a
6 TRCN0000102225 CTAGACGTAAAGTGACCTTAA pLKO.1 2622 3UTR 100% 10.800 8.640 N Lrrfip1 n/a
7 TRCN0000102226 GAACAGATTAAGAAGCTCAAA pLKO.1 1699 CDS 100% 4.950 3.960 N Lrrfip1 n/a
8 TRCN0000102227 CCCAGTCCCTAAGTAGAAGAT pLKO.1 725 CDS 100% 4.950 3.465 N Lrrfip1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006529213.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11348 pDONR223 100% 15.7% 16.9% None (many diffs) n/a
2 ccsbBroad304_11348 pLX_304 0% 15.7% 16.9% V5 (many diffs) n/a
3 TRCN0000466304 TGCATCGCAGTTCCCCTTGCGCGT pLX_317 100% 15.7% 16.9% V5 (many diffs) n/a
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