Transcript: Human NM_001099223.2

Homo sapiens intraflagellar transport 74 (IFT74), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-02-26
Taxon:
Homo sapiens (human)
Gene:
IFT74 (80173)
Length:
5384
CDS:
177..1979

Additional Resources:

NCBI RefSeq record:
NM_001099223.2
NBCI Gene record:
IFT74 (80173)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001099223.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000128272 GACAACTGATCTGGAGATATA pLKO.1 1607 CDS 100% 13.200 9.240 N IFT74 n/a
2 TRCN0000312500 GACAACTGATCTGGAGATATA pLKO_005 1607 CDS 100% 13.200 9.240 N IFT74 n/a
3 TRCN0000130561 GAGAGTGATTACCAGCCAATT pLKO.1 1881 CDS 100% 10.800 7.560 N IFT74 n/a
4 TRCN0000129824 CTGAAAGACAAGCGAAAGAAA pLKO.1 751 CDS 100% 5.625 3.938 N IFT74 n/a
5 TRCN0000312436 CTGAAAGACAAGCGAAAGAAA pLKO_005 751 CDS 100% 5.625 3.938 N IFT74 n/a
6 TRCN0000129758 CAAATCAGAAGTGTCGAAGAA pLKO.1 774 CDS 100% 4.950 3.465 N IFT74 n/a
7 TRCN0000312499 CAAATCAGAAGTGTCGAAGAA pLKO_005 774 CDS 100% 4.950 3.465 N IFT74 n/a
8 TRCN0000129251 CTGAAACGAAAGGCACAGATA pLKO.1 1323 CDS 100% 4.950 3.465 N IFT74 n/a
9 TRCN0000312438 CTGAAACGAAAGGCACAGATA pLKO_005 1323 CDS 100% 4.950 3.465 N IFT74 n/a
10 TRCN0000128802 CCAAGGTGAAATGAACCAGAA pLKO.1 1226 CDS 100% 4.050 2.835 N IFT74 n/a
11 TRCN0000113228 CGAGATCAAATGATTGCAGAA pLKO.1 1038 CDS 100% 4.050 2.835 N Ift74 n/a
12 TRCN0000288259 CGAGATCAAATGATTGCAGAA pLKO_005 1038 CDS 100% 4.050 2.835 N Ift74 n/a
13 TRCN0000128529 CGAAGAAGAAATTGAACAGGA pLKO.1 788 CDS 100% 2.640 1.584 N IFT74 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001099223.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04181 pDONR223 100% 61.2% 59.1% None (many diffs) n/a
2 ccsbBroad304_04181 pLX_304 0% 61.2% 59.1% V5 (many diffs) n/a
3 TRCN0000479761 TACTAGATATTTAGTTTGGGTCCC pLX_317 28.4% 61.2% 59.1% V5 (many diffs) n/a
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