Transcript: Human XM_017008019.2

PREDICTED: Homo sapiens F-box and leucine rich repeat protein 5 (FBXL5), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FBXL5 (26234)
Length:
3784
CDS:
1157..3067

Additional Resources:

NCBI RefSeq record:
XM_017008019.2
NBCI Gene record:
FBXL5 (26234)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017008019.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000284830 ATGCCTGTTTGCACGATTTAA pLKO_005 2322 CDS 100% 15.000 10.500 N FBXL5 n/a
2 TRCN0000272732 CAACGCAGCCAGACCATTTAT pLKO_005 1202 CDS 100% 15.000 10.500 N FBXL5 n/a
3 TRCN0000004291 GCTGAAGATTTGGCTGATATT pLKO.1 2441 CDS 100% 13.200 9.240 N FBXL5 n/a
4 TRCN0000349614 GCTGAAGATTTGGCTGATATT pLKO_005 2441 CDS 100% 13.200 9.240 N FBXL5 n/a
5 TRCN0000004292 GTACAGGAACAGCTTTAAGAA pLKO.1 2646 CDS 100% 5.625 3.938 N FBXL5 n/a
6 TRCN0000004290 CCTGATGATGAATGGGTGAAA pLKO.1 1799 CDS 100% 4.950 3.465 N FBXL5 n/a
7 TRCN0000272731 CCTGATGATGAATGGGTGAAA pLKO_005 1799 CDS 100% 4.950 3.465 N FBXL5 n/a
8 TRCN0000004293 GCACTTTACTCATAGCACATT pLKO.1 3122 3UTR 100% 4.950 3.465 N FBXL5 n/a
9 TRCN0000281896 GCACTTTACTCATAGCACATT pLKO_005 3122 3UTR 100% 4.950 3.465 N FBXL5 n/a
10 TRCN0000004294 GTCAGAACACTCCACAGGTAT pLKO.1 1585 CDS 100% 4.950 3.465 N FBXL5 n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 504 5UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 504 5UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017008019.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08004 pDONR223 100% 91.9% 91.8% None 0_1ins165;147A>G;559G>A n/a
2 ccsbBroad304_08004 pLX_304 0% 91.9% 91.8% V5 0_1ins165;147A>G;559G>A n/a
3 TRCN0000478007 TCGATCAACCTAAGCTTTCAGAGT pLX_317 18.5% 91.9% 91.8% V5 0_1ins165;147A>G;559G>A n/a
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