Transcript: Human NM_001271168.2

Homo sapiens PC-esterase domain containing 1A (PCED1A), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
PCED1A (64773)
Length:
1667
CDS:
308..1519

Additional Resources:

NCBI RefSeq record:
NM_001271168.2
NBCI Gene record:
PCED1A (64773)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001271168.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000130377 GAGACTGATCCACACATACAA pLKO.1 1453 CDS 100% 5.625 7.875 N PCED1A n/a
2 TRCN0000338738 GAGACTGATCCACACATACAA pLKO_005 1453 CDS 100% 5.625 7.875 N PCED1A n/a
3 TRCN0000128528 CTTCAACTATAATCCAGTGGA pLKO.1 1225 CDS 100% 2.640 3.696 N PCED1A n/a
4 TRCN0000129494 GAACTTCTACAGTGCTACGCT pLKO.1 793 CDS 100% 0.750 0.600 N PCED1A n/a
5 TRCN0000130992 CAGAAAGACTCACTGCTCACA pLKO.1 470 CDS 100% 2.640 1.848 N PCED1A n/a
6 TRCN0000338669 CAGAAAGACTCACTGCTCACA pLKO_005 470 CDS 100% 2.640 1.848 N PCED1A n/a
7 TRCN0000129522 GCTGCTACACAACAAGTTCGT pLKO.1 391 CDS 100% 2.640 1.848 N PCED1A n/a
8 TRCN0000338668 GCTGCTACACAACAAGTTCGT pLKO_005 391 CDS 100% 2.640 1.848 N PCED1A n/a
9 TRCN0000131185 GCTACACAACAAGTTCGTGGT pLKO.1 394 CDS 100% 2.160 1.512 N PCED1A n/a
10 TRCN0000129108 CACACATACAAACTGGACAGA pLKO.1 1463 CDS 100% 2.640 1.584 N PCED1A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001271168.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03965 pDONR223 100% 88.7% 88.7% None 202_203ins153 n/a
2 ccsbBroad304_03965 pLX_304 0% 88.7% 88.7% V5 202_203ins153 n/a
3 TRCN0000480929 CCAGCATTACTCGATATCGGTGCA pLX_317 28% 88.7% 88.7% V5 202_203ins153 n/a
4 ccsbBroadEn_12488 pDONR223 100% 77.3% 77.1% None 605C>T;635_907del n/a
5 ccsbBroad304_12488 pLX_304 0% 77.3% 77.1% V5 605C>T;635_907del n/a
6 TRCN0000471857 TTCTCTGACAGATAGAGAATCCTA pLX_317 42.7% 77.3% 77.1% V5 605C>T;635_907del n/a
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