Transcript: Human XM_024449929.1

PREDICTED: Homo sapiens Meis homeobox 2 (MEIS2), transcript variant X9, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MEIS2 (4212)
Length:
3065
CDS:
428..1594

Additional Resources:

NCBI RefSeq record:
XM_024449929.1
NBCI Gene record:
MEIS2 (4212)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024449929.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000274056 CCCACACCCTACTCAATTAAG pLKO_005 1723 3UTR 100% 13.200 18.480 N MEIS2 n/a
2 TRCN0000016046 CCACAAATCTCGCTGACCATA pLKO.1 1008 CDS 100% 4.950 6.930 N MEIS2 n/a
3 TRCN0000016043 CGGCCTTTGTTCCTCCATAAA pLKO.1 2463 3UTR 100% 13.200 10.560 N MEIS2 n/a
4 TRCN0000274057 TCTATGGGCACCCGTTGTTTC pLKO_005 627 CDS 100% 10.800 8.640 N MEIS2 n/a
5 TRCN0000310875 TCTATGGGCACCCGTTGTTTC pLKO_005 627 CDS 100% 10.800 8.640 N Meis2 n/a
6 TRCN0000016044 CCCATGATTGACCAGTCAAAT pLKO.1 1397 CDS 100% 13.200 9.240 N MEIS2 n/a
7 TRCN0000274060 CCCATGATTGACCAGTCAAAT pLKO_005 1397 CDS 100% 13.200 9.240 N MEIS2 n/a
8 TRCN0000274058 GAGCCAAGGAGCAGCATATAG pLKO_005 1447 CDS 100% 13.200 9.240 N MEIS2 n/a
9 TRCN0000274059 ACAAGCAATACAAGTACTAAG pLKO_005 832 CDS 100% 10.800 7.560 N MEIS2 n/a
10 TRCN0000016045 CCACCGATACATTAGCTGTTT pLKO.1 904 CDS 100% 4.950 3.465 N MEIS2 n/a
11 TRCN0000075590 CCCACAATGTTAAATTCTGTA pLKO.1 1853 3UTR 100% 4.950 3.465 N Meis2 n/a
12 TRCN0000301327 CCCACAATGTTAAATTCTGTA pLKO_005 1853 3UTR 100% 4.950 3.465 N Meis2 n/a
13 TRCN0000016047 CCAAGTAAACAACTGGTTTAT pLKO.1 1351 CDS 100% 1.320 0.924 N MEIS2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024449929.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00999 pDONR223 100% 98.1% 98.1% None 995_1015del n/a
2 ccsbBroad304_00999 pLX_304 0% 98.1% 98.1% V5 995_1015del n/a
3 TRCN0000470691 ACACACCCAATCGGACACGTGCAG pLX_317 35% 98.1% 98.1% V5 995_1015del n/a
4 ccsbBroadEn_00998 pDONR223 100% 78.3% 77.2% None (many diffs) n/a
5 ccsbBroad304_00998 pLX_304 0% 78.3% 77.2% V5 (many diffs) n/a
6 TRCN0000465719 ACTGCGGCTGATCAGATCCCCAGG pLX_317 23% 78.3% 77.2% V5 (many diffs) n/a
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