Transcript: Human NR_033328.2

Homo sapiens kelch like family member 7 (KLHL7), transcript variant 4, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
KLHL7 (55975)
Length:
5750
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_033328.2
NBCI Gene record:
KLHL7 (55975)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_033328.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000275529 AGCGAATGGACTGCTATAATG pLKO_005 1422 3UTR 100% 13.200 18.480 N KLHL7 n/a
2 TRCN0000249379 TACAAGCTGAACCACTTATTC pLKO_005 1113 3UTR 100% 13.200 10.560 N Klhl7 n/a
3 TRCN0000275530 TCATCAGTGAAGCGCAGTATC pLKO_005 2446 3UTR 100% 10.800 8.640 N KLHL7 n/a
4 TRCN0000249383 AGATGCTGAACCTGATATTAT pLKO_005 637 3UTR 100% 15.000 10.500 N Klhl7 n/a
5 TRCN0000134868 CTGCTAGAATTTCCGTGAATA pLKO.1 684 3UTR 100% 13.200 9.240 N KLHL7 n/a
6 TRCN0000275531 CTGCTAGAATTTCCGTGAATA pLKO_005 684 3UTR 100% 13.200 9.240 N KLHL7 n/a
7 TRCN0000137015 CGCAGTATCTTAGCTCTAGAT pLKO.1 2458 3UTR 100% 4.950 3.465 N KLHL7 n/a
8 TRCN0000137895 GAACTGAAAGCTGGCACACAA pLKO.1 1590 3UTR 100% 4.950 3.465 N KLHL7 n/a
9 TRCN0000137782 GCCTCTTTAGTCCTCACTGTT pLKO.1 2853 3UTR 100% 4.950 3.465 N KLHL7 n/a
10 TRCN0000136619 GCGAGTAACACATCTTCTCAA pLKO.1 937 3UTR 100% 4.950 3.465 N KLHL7 n/a
11 TRCN0000275532 GCGAGTAACACATCTTCTCAA pLKO_005 937 3UTR 100% 4.950 3.465 N KLHL7 n/a
12 TRCN0000138635 CCAACTCCAAAGTTCGTGCTT pLKO.1 2043 3UTR 100% 2.640 1.848 N KLHL7 n/a
13 TRCN0000275533 CCAACTCCAAAGTTCGTGCTT pLKO_005 2043 3UTR 100% 2.640 1.848 N KLHL7 n/a
14 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 3534 3UTR 100% 4.950 2.475 Y ERAP2 n/a
15 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 3535 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_033328.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15920 pDONR223 0% 7.2% None (many diffs) n/a
2 ccsbBroad304_15920 pLX_304 0% 7.2% V5 (many diffs) n/a
3 TRCN0000477622 CTAGAGAGATTTAGCCCGCTCTCA pLX_317 92% 7.2% V5 (many diffs) n/a
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