Transcript: Mouse XM_006533799.4

PREDICTED: Mus musculus dual specificity phosphatase 14 (Dusp14), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Mus musculus (mouse)
Gene:
Dusp14 (56405)
Length:
1850
CDS:
670..1266

Additional Resources:

NCBI RefSeq record:
XM_006533799.4
NBCI Gene record:
Dusp14 (56405)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006533799.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000226267 ACGCTGGGTGTTCGGGTTTAA pLKO_005 1370 3UTR 100% 13.200 10.560 N Dusp14 n/a
2 TRCN0000226265 GGCATCATCCCAGACGTTTAT pLKO_005 1204 CDS 100% 13.200 10.560 N Dusp14 n/a
3 TRCN0000030045 CCCGACACTTGATGCCTTATT pLKO.1 1235 CDS 100% 13.200 9.240 N Dusp14 n/a
4 TRCN0000226266 CCCGACACTTGATGCCTTATT pLKO_005 1235 CDS 100% 13.200 9.240 N Dusp14 n/a
5 TRCN0000030048 CTTTGGGAAGTCTTCAGTTAA pLKO.1 1164 CDS 100% 13.200 9.240 N Dusp14 n/a
6 TRCN0000218963 GTATTGCGTACCTGATGAAAT pLKO_005 1034 CDS 100% 13.200 9.240 N Dusp14 n/a
7 TRCN0000226264 ACTACGAGAGCCAGCTCTTTG pLKO_005 1148 CDS 100% 10.800 7.560 N Dusp14 n/a
8 TRCN0000030046 GAGGCATACAACTGGGTGAAA pLKO.1 1075 CDS 100% 4.950 3.465 N Dusp14 n/a
9 TRCN0000030047 TCAGATCACCTCCTCTCTCTT pLKO.1 753 CDS 100% 4.950 3.465 N Dusp14 n/a
10 TRCN0000030044 CCTCTGTATTGCGTACCTGAT pLKO.1 1029 CDS 100% 4.050 2.835 N Dusp14 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006533799.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02612 pDONR223 100% 90.7% 93.9% None (many diffs) n/a
2 ccsbBroad304_02612 pLX_304 0% 90.7% 93.9% V5 (many diffs) n/a
3 TRCN0000473282 AACTCTATAGGTTCATAGCCAAAT pLX_317 72.1% 90.7% 93.9% V5 (many diffs) n/a
4 TRCN0000488703 AAGGGGGGGTATCGTTCACAGGCA pLX_317 51.2% 90.7% 93.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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