Transcript: Human XM_005252072.2

PREDICTED: Homo sapiens AU RNA binding methylglutaconyl-CoA hydratase (AUH), transcript variant X7, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
AUH (549)
Length:
6936
CDS:
36..803

Additional Resources:

NCBI RefSeq record:
XM_005252072.2
NBCI Gene record:
AUH (549)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005252072.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000303617 GCAATAGATGGACTCGCTTTA pLKO_005 567 CDS 100% 10.800 8.640 N AUH n/a
2 TRCN0000303619 AGAGCAGTGATTAACGATATT pLKO_005 519 CDS 100% 13.200 9.240 N AUH n/a
3 TRCN0000303618 AGTGAAGTCCCAGGGATATTC pLKO_005 426 CDS 100% 13.200 9.240 N AUH n/a
4 TRCN0000052452 GATAAGAAAGTACGGACCATA pLKO.1 396 CDS 100% 4.950 3.465 N AUH n/a
5 TRCN0000052449 GCTTGGAATAAACAGAGCTTA pLKO.1 308 CDS 100% 4.950 3.465 N AUH n/a
6 TRCN0000299346 GCTTGGAATAAACAGAGCTTA pLKO_005 308 CDS 100% 4.950 3.465 N AUH n/a
7 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 2968 3UTR 100% 4.050 2.025 Y P3H4 n/a
8 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 2968 3UTR 100% 4.050 2.025 Y ORAI2 n/a
9 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 2968 3UTR 100% 4.050 2.025 Y P3H4 n/a
10 TRCN0000165704 CAATGGCACAATCTTGGCTCA pLKO.1 3601 3UTR 100% 2.160 1.080 Y LOC652276 n/a
11 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 3095 3UTR 100% 10.800 5.400 Y SMIM11A n/a
12 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 1178 3UTR 100% 5.625 2.813 Y KLHL30 n/a
13 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 1178 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005252072.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10692 pDONR223 100% 62.9% 56.7% None (many diffs) n/a
2 ccsbBroad304_10692 pLX_304 0% 62.9% 56.7% V5 (many diffs) n/a
3 TRCN0000481053 CAATGATTCCATAACCTCATAGTC pLX_317 32% 62.9% 56.7% V5 (many diffs) n/a
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