Construct: ORF TRCN0000481053

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004696.2_s317c1
Derived from:
ccsbBroadEn_10692
DNA Barcode:
CAATGATTCCATAACCTCATAGTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AUH (549)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481053
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 549 AUH AU RNA binding methylglutac... NM_001306190.1 100% 100%
2 human 549 AUH AU RNA binding methylglutac... XM_006717150.3 96.8% 96.5% 263_292del
3 human 549 AUH AU RNA binding methylglutac... NM_001698.2 91.4% 91.4% 417_503del
4 human 549 AUH AU RNA binding methylglutac... XM_005252066.3 88.8% 88.5% 263_292del;447_533del
5 human 549 AUH AU RNA binding methylglutac... XM_017014849.1 86.7% 86.7% 417_503del;892_893ins48
6 human 549 AUH AU RNA binding methylglutac... XM_005252067.4 84.2% 83.9% 263_292del;447_533del;922_923ins48
7 human 549 AUH AU RNA binding methylglutac... XM_005252069.4 73.7% 72.2% (many diffs)
8 human 549 AUH AU RNA binding methylglutac... XM_005252072.2 62.9% 56.7% (many diffs)
9 human 549 AUH AU RNA binding methylglutac... XM_011518800.3 61.1% 54.9% (many diffs)
10 human 549 AUH AU RNA binding methylglutac... NM_001351431.1 59.2% 59.2% 0_1ins327;90_176del
11 human 549 AUH AU RNA binding methylglutac... NM_001351432.1 59.2% 59.2% 0_1ins327;90_176del
12 human 549 AUH AU RNA binding methylglutac... NM_001351433.1 54.5% 54.5% 0_1ins327;90_176del;565_566ins48
13 human 549 AUH AU RNA binding methylglutac... XR_001746329.2 54% (many diffs)
14 human 549 AUH AU RNA binding methylglutac... XR_001746328.2 52.5% (many diffs)
15 human 549 AUH AU RNA binding methylglutac... XM_011518803.2 50.9% 45.2% (many diffs)
16 mouse 11992 Auh AU RNA binding protein/enoy... NM_016709.2 71.9% 78.7% (many diffs)
17 mouse 11992 Auh AU RNA binding protein/enoy... XM_011244372.2 51.2% 56.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
996
ORF length:
930
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggccgcggtg gcggcggcac ctggggcctt gggatccctg catgctggcg
121gcgcccgcct ggtggccgct tgcagtgcgt ggctctgccc ggggttgagg ctgcccggct
181cgttggcagg ccggcgagcg ggcccggcga tctgggccca gggctgggta cctgcggccg
241ggggtcccgc cccgaaaagg ggctacagct ctgagatgaa gacggaggac gagctgcggg
301tgcggcacct ggaggaggag aaccgaggaa ttgtggtgct tggaataaac agagcttatg
361gcaaaaattc actcagtaaa aatcttataa aaatgctatc aaaagctgtg gatgctttga
421aatctgataa gaaagtacgg accataataa tcaggagtga agtcccaggg atattctgtg
481ctgctaatct tccagtacca acaattgcag caatagatgg actcgcttta ggtggtggtc
541ttgaactggc tttagcctgt gatatacgag tagcagcttc ctctgcaaaa atgggcctgg
601ttgaaacaaa attggcgatt attcctggtg gaggggggac acagcgattg ccacgcgcca
661ttggaatgtc cctggccaag gagctcatat tctctgcgcg agTCCTCGAT GGCAAAGAAG
721CCAAAGCAGT GGGCTTAATC AGCCACGTTC TGGAACAGAA CCAGGAGGGA GACGCGGCCT
781ACAGGAAGGC CTTGGACCTG GCGAGAGAGT TTTTACCTCA GGGACCTGTT GCAATGAGAG
841TGGCAAAATT AGCAATTAAT CAAGGGATGG AGGTCGATTT AGTAACAGGG TTAGCCATAG
901AAGAAGCTTG TTATGCTCAG ACCATTCCAA CAAAAGACAG ACTTGAAGGT CTTCTTGCTT
961TTAAAGAGAA AAGGCCCCCT CGCTATAAAG GAGAATACCC AACTTTCTTG TACAAAGTGG
1021TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1081CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1141ACAATGATTC CATAACCTCA TAGTCACGCG TTAAGTCgac aatcaacctc tggattacaa
1201aatttgtgaa agatt

Download FASTA (ORF) (Full)