Construct: ORF TRCN0000481053
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004696.2_s317c1
- Derived from:
- ccsbBroadEn_10692
- DNA Barcode:
- CAATGATTCCATAACCTCATAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AUH (549)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481053
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 549 | AUH | AU RNA binding methylglutac... | NM_001306190.1 | 100% | 100% | |
2 | human | 549 | AUH | AU RNA binding methylglutac... | XM_006717150.3 | 96.8% | 96.5% | 263_292del |
3 | human | 549 | AUH | AU RNA binding methylglutac... | NM_001698.2 | 91.4% | 91.4% | 417_503del |
4 | human | 549 | AUH | AU RNA binding methylglutac... | XM_005252066.3 | 88.8% | 88.5% | 263_292del;447_533del |
5 | human | 549 | AUH | AU RNA binding methylglutac... | XM_017014849.1 | 86.7% | 86.7% | 417_503del;892_893ins48 |
6 | human | 549 | AUH | AU RNA binding methylglutac... | XM_005252067.4 | 84.2% | 83.9% | 263_292del;447_533del;922_923ins48 |
7 | human | 549 | AUH | AU RNA binding methylglutac... | XM_005252069.4 | 73.7% | 72.2% | (many diffs) |
8 | human | 549 | AUH | AU RNA binding methylglutac... | XM_005252072.2 | 62.9% | 56.7% | (many diffs) |
9 | human | 549 | AUH | AU RNA binding methylglutac... | XM_011518800.3 | 61.1% | 54.9% | (many diffs) |
10 | human | 549 | AUH | AU RNA binding methylglutac... | NM_001351431.1 | 59.2% | 59.2% | 0_1ins327;90_176del |
11 | human | 549 | AUH | AU RNA binding methylglutac... | NM_001351432.1 | 59.2% | 59.2% | 0_1ins327;90_176del |
12 | human | 549 | AUH | AU RNA binding methylglutac... | NM_001351433.1 | 54.5% | 54.5% | 0_1ins327;90_176del;565_566ins48 |
13 | human | 549 | AUH | AU RNA binding methylglutac... | XR_001746329.2 | 54% | (many diffs) | |
14 | human | 549 | AUH | AU RNA binding methylglutac... | XR_001746328.2 | 52.5% | (many diffs) | |
15 | human | 549 | AUH | AU RNA binding methylglutac... | XM_011518803.2 | 50.9% | 45.2% | (many diffs) |
16 | mouse | 11992 | Auh | AU RNA binding protein/enoy... | NM_016709.2 | 71.9% | 78.7% | (many diffs) |
17 | mouse | 11992 | Auh | AU RNA binding protein/enoy... | XM_011244372.2 | 51.2% | 56.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 996
- ORF length:
- 930
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccgcggtg gcggcggcac ctggggcctt gggatccctg catgctggcg 121 gcgcccgcct ggtggccgct tgcagtgcgt ggctctgccc ggggttgagg ctgcccggct 181 cgttggcagg ccggcgagcg ggcccggcga tctgggccca gggctgggta cctgcggccg 241 ggggtcccgc cccgaaaagg ggctacagct ctgagatgaa gacggaggac gagctgcggg 301 tgcggcacct ggaggaggag aaccgaggaa ttgtggtgct tggaataaac agagcttatg 361 gcaaaaattc actcagtaaa aatcttataa aaatgctatc aaaagctgtg gatgctttga 421 aatctgataa gaaagtacgg accataataa tcaggagtga agtcccaggg atattctgtg 481 ctgctaatct tccagtacca acaattgcag caatagatgg actcgcttta ggtggtggtc 541 ttgaactggc tttagcctgt gatatacgag tagcagcttc ctctgcaaaa atgggcctgg 601 ttgaaacaaa attggcgatt attcctggtg gaggggggac acagcgattg ccacgcgcca 661 ttggaatgtc cctggccaag gagctcatat tctctgcgcg agTCCTCGAT GGCAAAGAAG 721 CCAAAGCAGT GGGCTTAATC AGCCACGTTC TGGAACAGAA CCAGGAGGGA GACGCGGCCT 781 ACAGGAAGGC CTTGGACCTG GCGAGAGAGT TTTTACCTCA GGGACCTGTT GCAATGAGAG 841 TGGCAAAATT AGCAATTAAT CAAGGGATGG AGGTCGATTT AGTAACAGGG TTAGCCATAG 901 AAGAAGCTTG TTATGCTCAG ACCATTCCAA CAAAAGACAG ACTTGAAGGT CTTCTTGCTT 961 TTAAAGAGAA AAGGCCCCCT CGCTATAAAG GAGAATACCC AACTTTCTTG TACAAAGTGG 1021 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1081 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1141 ACAATGATTC CATAACCTCA TAGTCACGCG TTAAGTCgac aatcaacctc tggattacaa 1201 aatttgtgaa agatt