Construct: ORF TRCN0000477616
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010758.1_s317c1
- Derived from:
- ccsbBroadEn_12617
- DNA Barcode:
- GGTCTCAATTTCGCTGGCTGCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL36 (79786)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477616
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79786 | KLHL36 | kelch like family member 36 | NM_001303451.2 | 99.9% | 100% | 631C>T |
| 2 | human | 79786 | KLHL36 | kelch like family member 36 | NM_024731.4 | 89.7% | 89.7% | 631C>T;1105_1293del |
| 3 | human | 79786 | KLHL36 | kelch like family member 36 | XM_005256149.2 | 89.7% | 89.7% | 631C>T;1105_1293del |
| 4 | human | 79786 | KLHL36 | kelch like family member 36 | XR_001751986.2 | 22% | (many diffs) | |
| 5 | human | 79786 | KLHL36 | kelch like family member 36 | XR_933433.3 | 17.9% | (many diffs) | |
| 6 | mouse | 234796 | Klhl36 | kelch-like 36 | NM_146219.1 | 76.7% | 76.1% | (many diffs) |
| 7 | mouse | 234796 | Klhl36 | kelch-like 36 | XR_001778433.1 | 50.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1725
- ORF length:
- 1659
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaataag 61 ttggcatgat ggagggaagc aggcagacgc gagtgtctcg gccatacaag atcagcgaat 121 catcaaaggt ataccgctgg gccgaccact caagcacggt gctgcagcgg ctgaacgagc 181 agcgtctccg cgggctcttc tgcgacgtcg tcctggtggc cgatgagcag cgtgtgccag 241 cccatcgcaa cctgctggcc gtgtgcagcg actacttcaa ctccatgttc accatcggca 301 tgcgggaagc tttccagaag gaggtggagc tgatcggcgc ctcctacatt gggctcaagg 361 ccgtggtgga cttcctgtac ggcggggagc tggtgctgga tggcggcaac attgactacg 421 tcctggagac ggctcacctg ctgcagatct ggacggtggt agacttctgc tgtgagtacc 481 tggagcagga ggtgagcgag gacaactacc tgtacctgca ggagctggcc tccatctaca 541 gcctcaagcg gcttgatgcc ttcatcgatg gcttcatcct gaaccacttc ggcacgctgt 601 cctttacgcc cgacttcctg cagaacgtct ccatgcagaa gctgtgtgtc tacctgagca 661 gcagcgaggt gcagcgggag tgtgagcacg acctcttgca ggccgccctg cagtggctga 721 cgcagcagcc cgagcgcgag gcccacgccc gccaggtgct ggagaacatc cacttcccgc 781 tcatccccaa gaacgacctg ctgcaccgcg tcaagccggc cgtgtgctcg ctgctgccca 841 aggaggccaa ctgcgagggc ttcatcgagg aggccgtgcg ctaccacaac aacctggcgg 901 cccagcccgt catgcagacc aagcgcacgg cgctgcgcac caaccaggag cgcctgctgt 961 ttgtgggcgg cgaggtctcc gagcggtgtc tggagctcag tgacgacacc tgctacctgg 1021 acgccaagag cgagcagtgg gtcaaagaga cgccgctgcc cgcccggcgg agccaccact 1081 gtgtcgcggt gctggggggc ttcatcttca tcgccggcgg cagcttctca cgggacaacg 1141 gaggggatgc ggcctccaat cttctttata ggttcacgta cggccacgcg ggcaccatct 1201 acaaagactt cgtgtacatc tcggggggcc acgactacca aattggcccc taccgcaaga 1261 acctgctatg ctacgaccaC CGGACAGACG TGTGGGAGGA GCGGCGGCCC ATGACCACGG 1321 CGCGCGGCTG GCACAGCATG TGCAGCCTGG GTGACAGCAT CTACTCCATC GGGGGCAGCG 1381 ATGACAACAT CGAGTCCATG GAGCGCTTCG ACGTGCTGGG CGTGGAGGCC TACAGCCCGC 1441 AGTGCAACCA GTGGACCCGC GTGGCGCCGC TGCTGCACGC CAACAGCGAG TCGGGCGTGG 1501 CAGTGTGGGA GGGCCGCATC TACATCCTGG GCGGCTACAG CTGGGAGAAC ACTGCCTTCT 1561 CCAAGACCGT GCAGGTGTAC GACCGCGAGG CCGACAAGTG GAGCAGGGGC GTCGACCTGC 1621 CCAAGGCCAT CGCTGGCGGG TCCGCCTGTG TCTGCGCCCT GGAGCCACGG CCAGAGGACA 1681 AGAAGAAGAA AGGCAAAGGC AAGAGGCACC AGGACCGGGG CCAGTGCCCA ACTTTCTTGT 1741 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1801 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1861 GAAAGGACGA GGTCTCAATT TCGCTGGCTG CTTAACGCGT TAAGTCgaca atcaacctct 1921 ggattacaaa atttgtgaaa gatt