Transcript: Human XM_005258221.5

PREDICTED: Homo sapiens dystrobrevin alpha (DTNA), transcript variant X6, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DTNA (1837)
Length:
6578
CDS:
327..2468

Additional Resources:

NCBI RefSeq record:
XM_005258221.5
NBCI Gene record:
DTNA (1837)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005258221.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000336712 TTTCACCATCGATGCGAATAA pLKO_005 1625 CDS 100% 13.200 18.480 N DTNA n/a
2 TRCN0000336711 GATGTGTCCTGATGACTATAG pLKO_005 2659 3UTR 100% 10.800 15.120 N DTNA n/a
3 TRCN0000053910 CGGACTCAGTTTGAGGATCTT pLKO.1 2193 CDS 100% 4.950 6.930 N DTNA n/a
4 TRCN0000301131 CGGACTCAGTTTGAGGATCTT pLKO_005 2193 CDS 100% 4.950 6.930 N DTNA n/a
5 TRCN0000053909 CCTGCTAAGAAGCTGACTAAT pLKO.1 1209 CDS 100% 13.200 9.240 N DTNA n/a
6 TRCN0000301123 CCTGCTAAGAAGCTGACTAAT pLKO_005 1209 CDS 100% 13.200 9.240 N DTNA n/a
7 TRCN0000053912 GATGAAGAACACAGGCTAATT pLKO.1 1527 CDS 100% 13.200 9.240 N DTNA n/a
8 TRCN0000301124 GATGAAGAACACAGGCTAATT pLKO_005 1527 CDS 100% 13.200 9.240 N DTNA n/a
9 TRCN0000053908 CCCTTGACATACTTAATCATA pLKO.1 4463 3UTR 100% 5.625 3.938 N DTNA n/a
10 TRCN0000053911 CGATGCCAACAGTGTCACAAT pLKO.1 1095 CDS 100% 4.950 3.465 N DTNA n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005258221.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00464 pDONR223 100% 51.6% 50.6% None (many diffs) n/a
2 ccsbBroad304_00464 pLX_304 0% 51.6% 50.6% V5 (many diffs) n/a
3 TRCN0000474295 CCCAAGTAGACGGGTGTAGCACGA pLX_317 37.6% 51.6% 50.6% V5 (many diffs) n/a
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