Transcript: Human XM_005274594.5

PREDICTED: Homo sapiens zinc finger protein X-linked (ZFX), transcript variant X18, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZFX (7543)
Length:
7673
CDS:
618..2960

Additional Resources:

NCBI RefSeq record:
XM_005274594.5
NBCI Gene record:
ZFX (7543)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005274594.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000219096 TGTTATTGAGGACGTTGTTAT pLKO_005 740 CDS 100% 13.200 18.480 N ZFX n/a
2 TRCN0000234346 ACACGTCTTGACGGGTGATTC pLKO_005 935 CDS 100% 10.800 15.120 N ZFX n/a
3 TRCN0000221678 GTCGGAAATTGATCCTTGTAA pLKO.1 1247 CDS 100% 5.625 7.875 N ZFX n/a
4 TRCN0000221680 GACGTTGTTATAGAAGATGTT pLKO.1 750 CDS 100% 4.950 6.930 N ZFX n/a
5 TRCN0000234349 AGTCAGGTCTAATTATCATAA pLKO_005 3420 3UTR 100% 13.200 9.240 N ZFX n/a
6 TRCN0000234348 AGTTGGCCTGCCCTAACAATA pLKO_005 2945 CDS 100% 13.200 9.240 N ZFX n/a
7 TRCN0000221679 AGTGATTTGAAACGACACATA pLKO.1 2478 CDS 100% 4.950 3.465 N ZFX n/a
8 TRCN0000221677 CCAATCAGTCTCATTCACATA pLKO.1 3022 3UTR 100% 4.950 3.465 N ZFX n/a
9 TRCN0000221681 GCAGGATGATGACAAAGGCAA pLKO.1 1142 CDS 100% 2.640 1.848 N ZFX n/a
10 TRCN0000234347 TGGACACAGAGTCGGAAATTG pLKO_005 1237 CDS 100% 13.200 7.920 N ZFX n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005274594.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01791 pDONR223 100% 86.8% 86.9% None (many diffs) n/a
2 TRCN0000477192 CGGTAAATAATATAGGTCCGTATT pLX_317 20.2% 86.8% 86.9% V5 (many diffs) n/a
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