Transcript: Mouse XM_006501064.3

PREDICTED: Mus musculus rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2), transcript variant X6, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Arhgef2 (16800)
Length:
7639
CDS:
2037..4970

Additional Resources:

NCBI RefSeq record:
XM_006501064.3
NBCI Gene record:
Arhgef2 (16800)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006501064.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000432135 TGTCCGTGGAATCCCTTATTG pLKO_005 2566 CDS 100% 13.200 18.480 N Arhgef2 n/a
2 TRCN0000419564 AGCTAAAGATGCCCGCTATAC pLKO_005 2102 CDS 100% 10.800 15.120 N Arhgef2 n/a
3 TRCN0000109987 CGACGCTTTATACTTGAGCTT pLKO.1 4679 CDS 100% 2.640 3.696 N Arhgef2 n/a
4 TRCN0000427597 ACAGCCATTGATCAATCTTTA pLKO_005 4226 CDS 100% 13.200 9.240 N Arhgef2 n/a
5 TRCN0000109988 CCCTCATTTGTCCTACATGTA pLKO.1 2206 CDS 100% 4.950 3.465 N Arhgef2 n/a
6 TRCN0000109985 GCAGGAGATTTACAACCGAAT pLKO.1 3341 CDS 100% 4.050 2.835 N Arhgef2 n/a
7 TRCN0000109989 TCAGTGACATTCACACACGTT pLKO.1 2872 CDS 100% 2.640 1.848 N Arhgef2 n/a
8 TRCN0000166364 CACACACACACACACACACAA pLKO.1 1610 5UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006501064.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11345 pDONR223 100% 83% 86.8% None (many diffs) n/a
2 ccsbBroad304_11345 pLX_304 0% 83% 86.8% V5 (many diffs) n/a
3 TRCN0000468482 GATTAACGCATCTTACAAGATGTC pLX_317 13.8% 83% 86.8% V5 (many diffs) n/a
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