Transcript: Mouse XM_006501930.2

PREDICTED: Mus musculus pleckstrin homology domain containing, family O member 1 (Plekho1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Plekho1 (67220)
Length:
1389
CDS:
67..1269

Additional Resources:

NCBI RefSeq record:
XM_006501930.2
NBCI Gene record:
Plekho1 (67220)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006501930.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000339908 TCCGGAAATTCTGCGGGAAAG pLKO_005 122 CDS 100% 6.000 8.400 N Plekho1 n/a
2 TRCN0000189874 CCTCAGATGGGATGCTAACAT pLKO.1 578 CDS 100% 5.625 4.500 N Plekho1 n/a
3 TRCN0000339905 CCTCAGATGGGATGCTAACAT pLKO_005 578 CDS 100% 5.625 4.500 N Plekho1 n/a
4 TRCN0000202420 GAAATTCTGCGGGAAAGGGAT pLKO.1 126 CDS 100% 2.640 2.112 N Plekho1 n/a
5 TRCN0000192546 GCTAACATTAGACCTGATCCA pLKO.1 591 CDS 100% 2.640 2.112 N Plekho1 n/a
6 TRCN0000339977 ACGCCCTGAGTTCTGCCATTA pLKO_005 413 CDS 100% 10.800 7.560 N Plekho1 n/a
7 TRCN0000339975 AGTGCGAAGAGCTCCGGAAAT pLKO_005 269 CDS 100% 10.800 7.560 N Plekho1 n/a
8 TRCN0000189593 CTACCTCAGATGGGATGCTAA pLKO.1 575 CDS 100% 4.950 3.465 N Plekho1 n/a
9 TRCN0000190142 GTCCTGAAGAGAAGGAGTCAT pLKO.1 386 CDS 100% 4.950 3.465 N Plekho1 n/a
10 TRCN0000189875 CTTCAAGCTGAGGAATCCCTA pLKO.1 904 CDS 100% 2.640 1.848 N Plekho1 n/a
11 TRCN0000202056 CTTCACCTTCAAGCTGAGGAA pLKO.1 898 CDS 100% 2.640 1.848 N Plekho1 n/a
12 TRCN0000339906 CGTCTCCGAGAAGGAGGTAAA pLKO_005 201 CDS 100% 10.800 6.480 N Plekho1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006501930.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03238 pDONR223 100% 86.3% 88.8% None (many diffs) n/a
2 ccsbBroad304_03238 pLX_304 0% 86.3% 88.8% V5 (many diffs) n/a
3 TRCN0000466090 CTCTGTACCGTATTTAGTGGACCC pLX_317 16.1% 86.3% 88.8% V5 (many diffs) n/a
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