Transcript: Mouse XM_006505543.3

PREDICTED: Mus musculus G protein-coupled receptor 19 (Gpr19), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Gpr19 (14760)
Length:
3669
CDS:
2046..3551

Additional Resources:

NCBI RefSeq record:
XM_006505543.3
NBCI Gene record:
Gpr19 (14760)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006505543.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000328128 CTATGTGGGCATTTCGGAAAT pLKO_005 3413 CDS 100% 10.800 15.120 N Gpr19 n/a
2 TRCN0000328129 GCAGTCACGTGGGTATCTTTC pLKO_005 3243 CDS 100% 10.800 15.120 N Gpr19 n/a
3 TRCN0000028165 CCGCTTCTATACCATCGTCTA pLKO.1 2789 CDS 100% 4.050 5.670 N Gpr19 n/a
4 TRCN0000028162 CGCTTTGTGGTTGTTCTCCAT pLKO.1 2522 CDS 100% 2.640 3.696 N Gpr19 n/a
5 TRCN0000328126 CCACTCTGTACTCTATTTATA pLKO_005 3283 CDS 100% 15.000 10.500 N Gpr19 n/a
6 TRCN0000328127 TCTGCATGTCCTCGATGAAAT pLKO_005 3337 CDS 100% 13.200 9.240 N Gpr19 n/a
7 TRCN0000328193 TCTTGGTGGGCTTCGTGATTC pLKO_005 2986 CDS 100% 10.800 7.560 N Gpr19 n/a
8 TRCN0000028189 CCTGCTCTTGAACCTAGTGTT pLKO.1 3137 CDS 100% 4.950 3.465 N Gpr19 n/a
9 TRCN0000028174 CGTGGGTATCTTTCAGCTCTT pLKO.1 3250 CDS 100% 4.050 2.835 N Gpr19 n/a
10 TRCN0000028198 CCCACTCTGTACTCTATTTAT pLKO.1 3282 CDS 100% 15.000 9.000 N Gpr19 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006505543.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000487695 GAATTTATTGACATATCTATAATT pLX_317 21.8% 68.9% 74% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000487692 AAAGTACCAAGCGGCCGTTTACCG pLX_317 21.8% 68.8% 74% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000488209 ACGTATTACATCCCTGAGGGAATT pLX_317 29.4% 68.7% 73.7% V5 (many diffs) n/a
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