Construct: ORF TRCN0000487695

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021459.1_s317c1
DNA Barcode:
GAATTTATTGACATATCTATAATT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
GPR19 (2842)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487695
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2842 GPR19 G protein-coupled receptor 19 NM_006143.3 99.8% 99.7% 565G>A;840T>C
2 human 2842 GPR19 G protein-coupled receptor 19 XM_011520625.2 99.8% 99.7% 565G>A;840T>C
3 human 2842 GPR19 G protein-coupled receptor 19 XM_017019216.2 99.8% 99.7% 565G>A;840T>C
4 human 2842 GPR19 G protein-coupled receptor 19 XM_011520623.3 95.4% 95.3% 1_57del;622G>A;897T>C
5 human 2842 GPR19 G protein-coupled receptor 19 XM_011520624.2 95.4% 95.3% 1_57del;622G>A;897T>C
6 human 2842 GPR19 G protein-coupled receptor 19 XM_017019212.1 95.4% 95.3% 1_57del;622G>A;897T>C
7 human 2842 GPR19 G protein-coupled receptor 19 XM_017019213.1 95.4% 95.3% 1_57del;622G>A;897T>C
8 human 2842 GPR19 G protein-coupled receptor 19 XM_017019214.1 95.4% 95.3% 1_57del;622G>A;897T>C
9 mouse 14760 Gpr19 G protein-coupled receptor 19 NM_001167694.2 83.1% 89.3% (many diffs)
10 mouse 14760 Gpr19 G protein-coupled receptor 19 NM_001167697.2 83.1% 89.3% (many diffs)
11 mouse 14760 Gpr19 G protein-coupled receptor 19 NM_001167699.2 83.1% 89.3% (many diffs)
12 mouse 14760 Gpr19 G protein-coupled receptor 19 NM_001167695.2 81.7% 87.9% (many diffs)
13 mouse 14760 Gpr19 G protein-coupled receptor 19 NM_001167696.2 81.7% 87.9% (many diffs)
14 mouse 14760 Gpr19 G protein-coupled receptor 19 NM_001167700.2 81.7% 87.9% (many diffs)
15 mouse 14760 Gpr19 G protein-coupled receptor 19 NM_008157.3 81.7% 87.9% (many diffs)
16 mouse 14760 Gpr19 G protein-coupled receptor 19 XM_006505554.1 81.7% 87.9% (many diffs)
17 mouse 14760 Gpr19 G protein-coupled receptor 19 XM_017321393.1 81.7% 87.9% (many diffs)
18 mouse 14760 Gpr19 G protein-coupled receptor 19 XM_017321391.1 70.1% 75.4% (many diffs)
19 mouse 14760 Gpr19 G protein-coupled receptor 19 XM_017321392.1 70.1% 75.4% (many diffs)
20 mouse 14760 Gpr19 G protein-coupled receptor 19 XM_006505543.3 68.9% 74% (many diffs)
21 mouse 14760 Gpr19 G protein-coupled receptor 19 XM_006505544.3 68.9% 74% (many diffs)
22 mouse 14760 Gpr19 G protein-coupled receptor 19 XM_006505545.2 68.9% 74% (many diffs)
23 mouse 14760 Gpr19 G protein-coupled receptor 19 XM_006505547.3 68.9% 74% (many diffs)
24 mouse 14760 Gpr19 G protein-coupled receptor 19 XM_017321390.1 68.9% 74% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1317
ORF length:
1245
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggctccac catggttttt gctcacagaa tggataacag caagccacat ttgattattc
121ctacacttct ggtgcccctc caaaaccgca gctgcactga aacagccaca cctctgccaa
181gccaatacct gatggaatta agtgaggagc acagttggat gagcaaccaa acagaccttc
241actatgtgct gaaacccggg gaagtggcca cagccagcat cttctttggg attctgtggt
301tgttttctat cttcggcaat tccctggttt gtttggtcat ccataggagt aggaggactc
361agtctaccac caactacttt gtggtctcca tggcatgtgc tgaccttctc atcagcgttg
421ccagcacgcc tttcgtcctg ctccagttca ccactggaag gtggacgctg ggtagtgcaa
481cgtgcaaggt tgtgcgatat tttcaatatc tcactccagg tgtccagatc tacgttctcc
541tctccatctg catagaccgg ttctacacca tcgtctatcc tctgagcttc aaggtgtcca
601gagaaaaagc caagaaaatg attgcggcat cgtggatctt tgatgcaggc tttgtgaccc
661ctgtgctctt tttctatggc tccaactggg acagtcattg taactatttc ctcccctcct
721cttgggaagg cactgcctac actgtcatcc acttcttggt gggctttgtg attccatctg
781tcctcataat tttattttac caaaaggtca taaaatatat ttggagaata ggcacagatg
841gccgaacggt gaggaggaca atgaacattg tccctcggac aaaagtgaaa actatcaaga
901tgttcctcat cttaaatctg ttgtttttgc tctcctggct gccttttcat gtagctcagc
961tatggcaccc ccatgaacaa gactataaga aaagttccct tgttttcaca gctatcacat
1021ggatatcctt tagttcttca gcctctaaac cTACTCTGTA TTCAATTTAT AATGCCAATT
1081TTCGGAGAGG GATGAAAGAG ACTTTTTGCA TGTCCTCTAT GAAATGTTAC CGAAGCAATG
1141CCTATACTAT CACAACAAGT TCAAGGATGG CCAAAAAAAA CTACGTTGGC ATTTCAGAAA
1201TCCCTTCCAT GGCCAAAACT ATTACCAAAG ACTCGATCTA TGACTCATTT GACAGAGAAG
1261CCAAGGAAAA AAAGCTTGCT TGGCCCATTA ACTCAAATCC ACCAAATACT TTTGTCTAAG
1321ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1381GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1441TTTATATATC TTGTGGAAAG GACGAGAATT TATTGACATA TCTATAATTA CGCGTTAAGT
1501Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)

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