Transcript: Mouse XM_006506888.2

PREDICTED: Mus musculus pleckstrin homology domain containing, family A member 5 (Plekha5), transcript variant X33, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Plekha5 (109135)
Length:
4715
CDS:
801..3845

Additional Resources:

NCBI RefSeq record:
XM_006506888.2
NBCI Gene record:
Plekha5 (109135)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006506888.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000329300 ACGGGCAAGATAGACCATTAA pLKO_005 1048 CDS 100% 13.200 18.480 N Plekha5 n/a
2 TRCN0000192802 GCCTTTCTCATCAGTTAAGTA pLKO.1 2714 CDS 100% 5.625 7.875 N Plekha5 n/a
3 TRCN0000329298 GCCTTTCTCATCAGTTAAGTA pLKO_005 2714 CDS 100% 5.625 7.875 N Plekha5 n/a
4 TRCN0000192945 GCAGGTATTGATGCCAAATTA pLKO.1 2238 CDS 100% 15.000 10.500 N Plekha5 n/a
5 TRCN0000375339 CCTCCACTTCCTCGGTCTTAT pLKO_005 2769 CDS 100% 13.200 9.240 N Plekha5 n/a
6 TRCN0000190320 CCACACAAATGCTGTTGCTTA pLKO.1 4438 3UTR 100% 4.950 3.465 N Plekha5 n/a
7 TRCN0000190848 GCAGATTGTGTGAACAGGATA pLKO.1 2260 CDS 100% 4.950 3.465 N Plekha5 n/a
8 TRCN0000190624 GCTGTTGCTTAGCTTTCCTTT pLKO.1 4448 3UTR 100% 4.950 3.465 N Plekha5 n/a
9 TRCN0000190596 GCAGTGGATTAAAGTCCAGAA pLKO.1 1298 CDS 100% 4.050 2.835 N Plekha5 n/a
10 TRCN0000329364 GCAGTGGATTAAAGTCCAGAA pLKO_005 1298 CDS 100% 4.050 2.835 N Plekha5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006506888.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12044 pDONR223 100% 67.2% 66.9% None (many diffs) n/a
2 ccsbBroad304_12044 pLX_304 0% 67.2% 66.9% V5 (many diffs) n/a
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