Transcript: Mouse XM_006508279.2

PREDICTED: Mus musculus anaphase prompoting complex C subunit 15 (Anapc15), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Anapc15 (75430)
Length:
1620
CDS:
352..837

Additional Resources:

NCBI RefSeq record:
XM_006508279.2
NBCI Gene record:
Anapc15 (75430)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006508279.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000181918 CATCGCAGAGAAAGACAACAA pLKO.1 513 CDS 100% 4.950 3.465 N Anapc15 n/a
2 TRCN0000292794 CATCGCAGAGAAAGACAACAA pLKO_005 513 CDS 100% 4.950 3.465 N Anapc15 n/a
3 TRCN0000181534 GCAAGACATGGATGAGATGAA pLKO.1 642 CDS 100% 4.950 3.465 N Anapc15 n/a
4 TRCN0000292850 GCAAGACATGGATGAGATGAA pLKO_005 642 CDS 100% 4.950 3.465 N Anapc15 n/a
5 TRCN0000177202 GACTATAATGAGTCACCTGAT pLKO.1 664 CDS 100% 4.050 2.835 N Anapc15 n/a
6 TRCN0000292851 GACTATAATGAGTCACCTGAT pLKO_005 664 CDS 100% 4.050 2.835 N Anapc15 n/a
7 TRCN0000181627 GAAGATGATGAGGACATGCAA pLKO.1 625 CDS 100% 3.000 2.100 N Anapc15 n/a
8 TRCN0000182465 GACAGTGAAGAGGATTCCGAA pLKO.1 607 CDS 100% 2.640 1.848 N Anapc15 n/a
9 TRCN0000181505 GAGAAAGACAACAACCTGGTA pLKO.1 520 CDS 100% 2.640 1.848 N Anapc15 n/a
10 TRCN0000144934 GAAGAGGATGATGAAGATGAT pLKO.1 583 CDS 100% 4.950 2.475 Y ANAPC15 n/a
11 TRCN0000344368 GAAGAGGATGATGAAGATGAT pLKO_005 583 CDS 100% 4.950 2.475 Y ANAPC15 n/a
12 TRCN0000143366 GAGAAAGACAACAACCTGGTT pLKO.1 520 CDS 100% 2.640 1.848 N ANAPC15 n/a
13 TRCN0000344428 GAGAAAGACAACAACCTGGTT pLKO_005 520 CDS 100% 2.640 1.848 N ANAPC15 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006508279.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02882 pDONR223 100% 69.1% 66.4% None (many diffs) n/a
2 ccsbBroad304_02882 pLX_304 0% 69.1% 66.4% V5 (many diffs) n/a
3 TRCN0000475223 CAATCCACTCCAGTTATACAATTA pLX_317 91.1% 69.1% 66.4% V5 (many diffs) n/a
4 ccsbBroadEn_07963 pDONR223 100% 68.9% 66.4% None (many diffs) n/a
5 ccsbBroad304_07963 pLX_304 0% 68.9% 66.4% V5 (many diffs) n/a
6 TRCN0000467174 AGGCTCACCGTTGGTGCGTTGACA pLX_317 83.5% 68.9% 66.4% V5 (many diffs) n/a
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