Transcript: Mouse XM_006511027.3

PREDICTED: Mus musculus transducin-like enhancer of split 3 (Tle3), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Tle3 (21887)
Length:
4166
CDS:
1113..3437

Additional Resources:

NCBI RefSeq record:
XM_006511027.3
NBCI Gene record:
Tle3 (21887)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006511027.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000444848 TACGTGAAGGACGGCAGTTAC pLKO_005 3058 CDS 100% 10.800 15.120 N Tle3 n/a
2 TRCN0000097968 GCGGATGACAAATATATTGTA pLKO.1 3369 CDS 100% 5.625 7.875 N Tle3 n/a
3 TRCN0000097965 GACTCTAACAGGCCTGTCAAA pLKO.1 3443 3UTR 100% 4.950 6.930 N Tle3 n/a
4 TRCN0000097966 GCGCCATTATGTGATGTACTA pLKO.1 1286 CDS 100% 4.950 6.930 N Tle3 n/a
5 TRCN0000097967 CCACACTAAGCCCGACAAATA pLKO.1 3179 CDS 100% 13.200 10.560 N Tle3 n/a
6 TRCN0000439755 AGATGTCCTATGGCTTGAATA pLKO_005 1309 CDS 100% 13.200 9.240 N Tle3 n/a
7 TRCN0000443843 TGACAAGAAGGCCACAGTTTA pLKO_005 3401 CDS 100% 13.200 9.240 N Tle3 n/a
8 TRCN0000439719 GCGAAGAGACTGAACACAATC pLKO_005 1356 CDS 100% 10.800 7.560 N Tle3 n/a
9 TRCN0000019689 CGCCATTATGTGATGTACTAT pLKO.1 1287 CDS 100% 5.625 3.938 N TLE3 n/a
10 TRCN0000097969 CTCAGCAGATACGATAGCGAT pLKO.1 1845 CDS 100% 2.640 1.848 N Tle3 n/a
11 TRCN0000442749 GGTTTGTGAACAGTGTAAATA pLKO_005 3839 3UTR 100% 15.000 9.000 N Tle3 n/a
12 TRCN0000443070 CCAAAGACCTTGGTCATAATG pLKO_005 2044 CDS 100% 13.200 7.920 N Tle3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006511027.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11191 pDONR223 100% 88.4% 95.5% None (many diffs) n/a
2 ccsbBroad304_11191 pLX_304 0% 88.4% 95.5% V5 (many diffs) n/a
3 TRCN0000479195 CTTACGAATAACTTTCACCGCCGG pLX_317 14.5% 88.4% 95.5% V5 (many diffs) n/a
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