Construct: ORF TRCN0000489197
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020035.1_s317c1
- DNA Barcode:
- GGTACACCCGTGTGGTACCCGATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRM1 (2911)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489197
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2911 | GRM1 | glutamate metabotropic rece... | NM_001278064.2 | 99.9% | 99.9% | 3168T>G;3582_3583insG |
| 2 | human | 2911 | GRM1 | glutamate metabotropic rece... | XM_011535782.1 | 99.9% | 99.9% | 3168T>G;3582_3583insG |
| 3 | human | 2911 | GRM1 | glutamate metabotropic rece... | XM_017010783.1 | 99.9% | 99.9% | 3168T>G;3582_3583insG |
| 4 | human | 2911 | GRM1 | glutamate metabotropic rece... | XM_017010784.1 | 99.9% | 99.9% | 3168T>G;3582_3583insG |
| 5 | human | 2911 | GRM1 | glutamate metabotropic rece... | NM_001278067.1 | 76% | 74.5% | 2660_2661ins35;2724_2725ins824 |
| 6 | human | 2911 | GRM1 | glutamate metabotropic rece... | NM_001278065.2 | 74.9% | 74.6% | (many diffs) |
| 7 | human | 2911 | GRM1 | glutamate metabotropic rece... | NM_001278066.1 | 74.9% | 74.6% | (many diffs) |
| 8 | human | 2911 | GRM1 | glutamate metabotropic rece... | XM_017010785.1 | 74.9% | 74.6% | (many diffs) |
| 9 | human | 2911 | GRM1 | glutamate metabotropic rece... | XM_017010786.1 | 74.9% | 74.6% | (many diffs) |
| 10 | human | 2911 | GRM1 | glutamate metabotropic rece... | XM_017010787.1 | 74.9% | 74.6% | (many diffs) |
| 11 | human | 2911 | GRM1 | glutamate metabotropic rece... | XM_024446412.1 | 74.9% | 74.6% | (many diffs) |
| 12 | human | 2911 | GRM1 | glutamate metabotropic rece... | XM_017010788.1 | 65.6% | 65.6% | 0_1ins1227;1941T>G;2355_2356insG |
| 13 | mouse | 14816 | Grm1 | glutamate receptor, metabot... | NM_016976.3 | 87.4% | 93.9% | (many diffs) |
| 14 | mouse | 14816 | Grm1 | glutamate receptor, metabot... | XM_006512549.3 | 87.4% | 93.9% | (many diffs) |
| 15 | mouse | 14816 | Grm1 | glutamate receptor, metabot... | XM_006512550.3 | 69.6% | 74.7% | (many diffs) |
| 16 | mouse | 14816 | Grm1 | glutamate receptor, metabot... | XM_006512551.3 | 67.3% | 71.6% | (many diffs) |
| 17 | mouse | 14816 | Grm1 | glutamate receptor, metabot... | NM_001114333.2 | 66.8% | 72.8% | (many diffs) |
| 18 | mouse | 14816 | Grm1 | glutamate receptor, metabot... | XM_006512554.3 | 57.1% | 60.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3654
- ORF length:
- 3585
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggtcgggctc cttttgtttt ttttcccagc gatctttttg gaggtgtccc 121 ttctccccag aagccccggc aggaaagtgt tgctggcagg agcgtcgtct cagcgctcgg 181 tggccagaat ggacggagat gtcatcattg gagccctctt ctcagtccat caccagcctc 241 cggccgagaa agtgcccgag aggaagtgtg gggagatcag ggagcagtat ggcatccaga 301 gggtggaggc catgttccac acgttggata agatcaacgc ggacccggtc ctcctgccca 361 acatcaccct gggcagtgag atccgggact cctgctggca ctcttccgtg gctctggaac 421 agagcattga gttcattagg gactctctga tttccattcg agatgagaag gatgggatca 481 accggtgtct gcctgacggc cagtccctcc ccccaggcag gactaagaag cccattgcgg 541 gagtgatcgg tcccggctcc agctctgtag ccattcaagt gcagaacctg ctccagctct 601 tcgacatccc ccagatcgct tattcagcca caagcatcga cctgagtgac aaaactttgt 661 acaaatactt cctgagggtt gtcccttctg acactttgca ggcaagggcc atgcttgaca 721 tagtcaaacg ttacaattgg acctatgtct ctgcagtcca cacggaaggg aattatgggg 781 agagcggaat ggacgctttc aaagagctgg ctgcccagga aggcctctgt atcgcccatt 841 ctgacaaaat ctacagcaac gctggggaga agagctttga ccgactcttg cgcaaactcc 901 gagagaggct tcccaaggct agagtggtgg tctgcttctg tgaaggcatg acagtgcgag 961 gactcctgag cgccatgcgg cgccttggcg tcgtgggcga gttctcactc attggaagtg 1021 atggatgggc agacagagat gaagtcattg aaggttatga ggtggaagcc aacgggggaa 1081 tcacgataaa gctgcagtct ccagaggtca ggtcatttga tgattatttc ctgaaactga 1141 ggctggacac taacacgagg aatccctggt tccctgagtt ctggcaacat cggttccagt 1201 gccgccttcc aggacacctt ctggaaaatc ccaactttaa acgaatctgc acaggcaatg 1261 aaagcttaga agaaaactat gtccaggaca gtaagatggg gtttgtcatc aatgccatct 1321 atgccatggc acatgggctg cagaacatgc accatgccct ctgccctggc cacgtgggcc 1381 tctgcgatgc catgaagccc atcgacggca gcaagctgct ggacttcctc atcaagtcct 1441 cattcattgg agtatctgga gaggaggtgt ggtttgatga gaaaggagac gctcctggaa 1501 ggtatgatat catgaatctg cagtacactg aagctaatcg ctatgactat gtgcacgttg 1561 gaacctggca tgaaggagtg ctgaacattg atgattacaa aatccagatg aacaagagtg 1621 gagtggtgcg gtctgtgtgc agtgagcctt gcttaaaggg ccagattaag gttatacgga 1681 aaggagaagt gagctgctgc tggatttgca cggcctgcaa agagaatgaa tatgtgcaag 1741 atgagttcac ctgcaaagct tgtgacttgg gatggtggcc caatgcagat ctaacaggct 1801 gtgagcccat tcctgtgcgc tatcttgagt ggagcaacat cgaatccatt atagccatcg 1861 ccttttcatg cctgggaatc cttgttacct tgtttgtcac cctaatcttt gtactgtacc 1921 gggacacacc agtggtcaaa tcctccagtc gggagctctg ctacatcatc ctagctggca 1981 tcttccttgg ttatgtgtgc ccattcactc tcattgccaa acctactacc acctcctgct 2041 acctccagcg cctcttggtt ggcctctcct ctgcgatgtg ctactctgct ttagtgacta 2101 aaaccaatcg tattgcacgc atcctggctg gcagcaagaa gaagatctgc acccggaagc 2161 ccaggttcat gagtgcctgg gctcaggtga tcattgcctc aattctgatt agtgtgcaac 2221 taaccctggt ggtaaccctg atcatcatgg aaccccctat gcccattctg tcctacccaa 2281 gtatcaagga agtctacctt atctgcaata ccagcaacct gggtgtggtg gcccctttgg 2341 gctacaatgg actcctcatc atgagctgta cctactatgc cttcaagacc cgcaacgtgc 2401 ccgccaactt caacgaggcc aaatatatcg cgttcaccat gtacaccacc tgtatcatct 2461 ggctagcttt tgtgcccatt tactttggga gcaactacaa gatcatcaca acttgctttg 2521 cagtgagtct cagtgtaaca gtggctctgg ggtgcatgtt cactcccaag atgtacatca 2581 ttattgccaa gcctgagagg aatgtccgca gtgccttcac cacctctgat gttgtccgca 2641 tgcatgttgg cgatggcaag ctgccctgcc gctccaacac tttcctcaac atcttccgaa 2701 gaaagaaggc aggggcaggg aatgccaatt ctaatggcaa gtctgtgtca tggtctgaac 2761 caggtggagg acaggtgccc aagggacagc atatgtggca ccgcctctct gtgcacgtga 2821 agaccaatga gacggcctgc aaccaaacag ccgtcatcaa gcccctcact aaaagttacc 2881 aaggctctgg caagagcctg accttttcag ataccagcac caagaccctt tacaacgtag 2941 aggaggagga ggatgcccag ccgattcgct ttagcccgcc tggtagccct tccatggtgg 3001 tgcacaggcg cgtgccaagc gcggcgacca ctccgcctct gccgtcccac ctgaccgcag 3061 aggagacccc cctcttcctg gccgaaccag ccctccccaa gggcttgccc cctcctctcc 3121 agcagcagca gcaaccccct ccacagcaga aatcgctgat ggaccagctc cagggagtgg 3181 tcagcaactt cagtaccgcg atcccggatt ttcacgcggt gctggcaggc cccgggggtc 3241 ccgggaacgg gctgcggtcc ctgtacccgc ccccgccacc tccgcagcac ctgcagatgc 3301 tgccgctgca gctgagcacc tttGGGGAGG AGCTGGTCTC CCCGCCCGCG GACGACGACG 3361 ACGACAGCGA GAGGTTTAAG CTCCTCCAGG AGTACGTGTA TGAGCACGAG CGGGAAGGGA 3421 ACACGGAAGA AGACGAACTG GAAGAGGAGG AGGAGGACCT GCAGGCGGCC AGCAAACTGA 3481 CCCCGGATGA TTCGCCTGCG CTGACGCCTC CGTCGCCTTT CCGCGACTCG GTGGCCTCGG 3541 GCAGCTCGGT GCCCAGCTCC CCCGTGTCCG AGTCGGTGCT CTGCACCCCT CCCAACGTAT 3601 CCTACGCCTC TGTCATTCTG CGGGACTACA AGCAAAGCTC TTCCACCCTG GACCCAGCTT 3661 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 3721 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 3781 CTTGTGGAAA GGACGAGGTA CACCCGTGTG GTACCCGATA ACGCGTTAAG TCgacaatca 3841 acctctggat tacaaaattt gtgaaagatt