Construct: ORF TRCN0000489197

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020035.1_s317c1
DNA Barcode:
GGTACACCCGTGTGGTACCCGATA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRM1 (2911)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489197
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2911 GRM1 glutamate metabotropic rece... NM_001278064.2 99.9% 99.9% 3168T>G;3582_3583insG
2 human 2911 GRM1 glutamate metabotropic rece... XM_011535782.1 99.9% 99.9% 3168T>G;3582_3583insG
3 human 2911 GRM1 glutamate metabotropic rece... XM_017010783.1 99.9% 99.9% 3168T>G;3582_3583insG
4 human 2911 GRM1 glutamate metabotropic rece... XM_017010784.1 99.9% 99.9% 3168T>G;3582_3583insG
5 human 2911 GRM1 glutamate metabotropic rece... NM_001278067.1 76% 74.5% 2660_2661ins35;2724_2725ins824
6 human 2911 GRM1 glutamate metabotropic rece... NM_001278065.2 74.9% 74.6% (many diffs)
7 human 2911 GRM1 glutamate metabotropic rece... NM_001278066.1 74.9% 74.6% (many diffs)
8 human 2911 GRM1 glutamate metabotropic rece... XM_017010785.1 74.9% 74.6% (many diffs)
9 human 2911 GRM1 glutamate metabotropic rece... XM_017010786.1 74.9% 74.6% (many diffs)
10 human 2911 GRM1 glutamate metabotropic rece... XM_017010787.1 74.9% 74.6% (many diffs)
11 human 2911 GRM1 glutamate metabotropic rece... XM_024446412.1 74.9% 74.6% (many diffs)
12 human 2911 GRM1 glutamate metabotropic rece... XM_017010788.1 65.6% 65.6% 0_1ins1227;1941T>G;2355_2356insG
13 mouse 14816 Grm1 glutamate receptor, metabot... NM_016976.3 87.4% 93.9% (many diffs)
14 mouse 14816 Grm1 glutamate receptor, metabot... XM_006512549.3 87.4% 93.9% (many diffs)
15 mouse 14816 Grm1 glutamate receptor, metabot... XM_006512550.3 69.6% 74.7% (many diffs)
16 mouse 14816 Grm1 glutamate receptor, metabot... XM_006512551.3 67.3% 71.6% (many diffs)
17 mouse 14816 Grm1 glutamate receptor, metabot... NM_001114333.2 66.8% 72.8% (many diffs)
18 mouse 14816 Grm1 glutamate receptor, metabot... XM_006512554.3 57.1% 60.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3654
ORF length:
3585
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat ggtcgggctc cttttgtttt ttttcccagc gatctttttg gaggtgtccc
121ttctccccag aagccccggc aggaaagtgt tgctggcagg agcgtcgtct cagcgctcgg
181tggccagaat ggacggagat gtcatcattg gagccctctt ctcagtccat caccagcctc
241cggccgagaa agtgcccgag aggaagtgtg gggagatcag ggagcagtat ggcatccaga
301gggtggaggc catgttccac acgttggata agatcaacgc ggacccggtc ctcctgccca
361acatcaccct gggcagtgag atccgggact cctgctggca ctcttccgtg gctctggaac
421agagcattga gttcattagg gactctctga tttccattcg agatgagaag gatgggatca
481accggtgtct gcctgacggc cagtccctcc ccccaggcag gactaagaag cccattgcgg
541gagtgatcgg tcccggctcc agctctgtag ccattcaagt gcagaacctg ctccagctct
601tcgacatccc ccagatcgct tattcagcca caagcatcga cctgagtgac aaaactttgt
661acaaatactt cctgagggtt gtcccttctg acactttgca ggcaagggcc atgcttgaca
721tagtcaaacg ttacaattgg acctatgtct ctgcagtcca cacggaaggg aattatgggg
781agagcggaat ggacgctttc aaagagctgg ctgcccagga aggcctctgt atcgcccatt
841ctgacaaaat ctacagcaac gctggggaga agagctttga ccgactcttg cgcaaactcc
901gagagaggct tcccaaggct agagtggtgg tctgcttctg tgaaggcatg acagtgcgag
961gactcctgag cgccatgcgg cgccttggcg tcgtgggcga gttctcactc attggaagtg
1021atggatgggc agacagagat gaagtcattg aaggttatga ggtggaagcc aacgggggaa
1081tcacgataaa gctgcagtct ccagaggtca ggtcatttga tgattatttc ctgaaactga
1141ggctggacac taacacgagg aatccctggt tccctgagtt ctggcaacat cggttccagt
1201gccgccttcc aggacacctt ctggaaaatc ccaactttaa acgaatctgc acaggcaatg
1261aaagcttaga agaaaactat gtccaggaca gtaagatggg gtttgtcatc aatgccatct
1321atgccatggc acatgggctg cagaacatgc accatgccct ctgccctggc cacgtgggcc
1381tctgcgatgc catgaagccc atcgacggca gcaagctgct ggacttcctc atcaagtcct
1441cattcattgg agtatctgga gaggaggtgt ggtttgatga gaaaggagac gctcctggaa
1501ggtatgatat catgaatctg cagtacactg aagctaatcg ctatgactat gtgcacgttg
1561gaacctggca tgaaggagtg ctgaacattg atgattacaa aatccagatg aacaagagtg
1621gagtggtgcg gtctgtgtgc agtgagcctt gcttaaaggg ccagattaag gttatacgga
1681aaggagaagt gagctgctgc tggatttgca cggcctgcaa agagaatgaa tatgtgcaag
1741atgagttcac ctgcaaagct tgtgacttgg gatggtggcc caatgcagat ctaacaggct
1801gtgagcccat tcctgtgcgc tatcttgagt ggagcaacat cgaatccatt atagccatcg
1861ccttttcatg cctgggaatc cttgttacct tgtttgtcac cctaatcttt gtactgtacc
1921gggacacacc agtggtcaaa tcctccagtc gggagctctg ctacatcatc ctagctggca
1981tcttccttgg ttatgtgtgc ccattcactc tcattgccaa acctactacc acctcctgct
2041acctccagcg cctcttggtt ggcctctcct ctgcgatgtg ctactctgct ttagtgacta
2101aaaccaatcg tattgcacgc atcctggctg gcagcaagaa gaagatctgc acccggaagc
2161ccaggttcat gagtgcctgg gctcaggtga tcattgcctc aattctgatt agtgtgcaac
2221taaccctggt ggtaaccctg atcatcatgg aaccccctat gcccattctg tcctacccaa
2281gtatcaagga agtctacctt atctgcaata ccagcaacct gggtgtggtg gcccctttgg
2341gctacaatgg actcctcatc atgagctgta cctactatgc cttcaagacc cgcaacgtgc
2401ccgccaactt caacgaggcc aaatatatcg cgttcaccat gtacaccacc tgtatcatct
2461ggctagcttt tgtgcccatt tactttggga gcaactacaa gatcatcaca acttgctttg
2521cagtgagtct cagtgtaaca gtggctctgg ggtgcatgtt cactcccaag atgtacatca
2581ttattgccaa gcctgagagg aatgtccgca gtgccttcac cacctctgat gttgtccgca
2641tgcatgttgg cgatggcaag ctgccctgcc gctccaacac tttcctcaac atcttccgaa
2701gaaagaaggc aggggcaggg aatgccaatt ctaatggcaa gtctgtgtca tggtctgaac
2761caggtggagg acaggtgccc aagggacagc atatgtggca ccgcctctct gtgcacgtga
2821agaccaatga gacggcctgc aaccaaacag ccgtcatcaa gcccctcact aaaagttacc
2881aaggctctgg caagagcctg accttttcag ataccagcac caagaccctt tacaacgtag
2941aggaggagga ggatgcccag ccgattcgct ttagcccgcc tggtagccct tccatggtgg
3001tgcacaggcg cgtgccaagc gcggcgacca ctccgcctct gccgtcccac ctgaccgcag
3061aggagacccc cctcttcctg gccgaaccag ccctccccaa gggcttgccc cctcctctcc
3121agcagcagca gcaaccccct ccacagcaga aatcgctgat ggaccagctc cagggagtgg
3181tcagcaactt cagtaccgcg atcccggatt ttcacgcggt gctggcaggc cccgggggtc
3241ccgggaacgg gctgcggtcc ctgtacccgc ccccgccacc tccgcagcac ctgcagatgc
3301tgccgctgca gctgagcacc tttGGGGAGG AGCTGGTCTC CCCGCCCGCG GACGACGACG
3361ACGACAGCGA GAGGTTTAAG CTCCTCCAGG AGTACGTGTA TGAGCACGAG CGGGAAGGGA
3421ACACGGAAGA AGACGAACTG GAAGAGGAGG AGGAGGACCT GCAGGCGGCC AGCAAACTGA
3481CCCCGGATGA TTCGCCTGCG CTGACGCCTC CGTCGCCTTT CCGCGACTCG GTGGCCTCGG
3541GCAGCTCGGT GCCCAGCTCC CCCGTGTCCG AGTCGGTGCT CTGCACCCCT CCCAACGTAT
3601CCTACGCCTC TGTCATTCTG CGGGACTACA AGCAAAGCTC TTCCACCCTG GACCCAGCTT
3661TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
3721CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
3781CTTGTGGAAA GGACGAGGTA CACCCGTGTG GTACCCGATA ACGCGTTAAG TCgacaatca
3841acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)